高浓度葡萄糖对培养血管内皮细胞活力和黏附分子表达的影响  

Effects of High Glucose Concentration on Adhesion Molecules Expression and Survival in Cultured Human Umbilical Vein Endothelial Cells

作　　者：穆王君 史斌[1] 

机构地区：[1]首都医科大学附属复兴医院内科

出　　处：《首都医科大学学报》2005年第3期315-318,共4页Journal of Capital Medical University

摘　　要：目的探讨高浓度葡萄糖作用于脐静脉内皮细胞后,对脐静脉血管内皮细胞系的活力和白细胞黏附分子〔血管细胞黏附分子-1(VCAM-1)、细胞间黏附分子-1(ICAM-1)〕表达的影响,为阐明高血糖在糖尿病血管病变的早期发病中作用与机制提供依据。方法采用胎盘蓝染色法及流式细胞仪检测细胞活力和黏附分子表达。结果高浓度葡萄糖可使内皮细胞的死亡率增加,ICAM-1表达显著上调,但VCAM-1表达无明显变化。结论减少高糖诱导的ICAM-1、VCAM-1表达增加,对减少糖尿病患者慢性并发症的发生概率是一条有益的途径。Objective To investigate whether or not survival was decreased after incubation with high glucose concentrations in culture media, and to examine the effects of glucose on the expression of intercellular adhesion molecule-1(ICAM-1)?vascular cell adhesion molecule(VCAM-1) in cultured human umbilical vein endothelial cells. Methods Human umbilical vein endothelial cells (ECV304) were incubated in a culture medium with 11.2 mmol/L, 16.8 mmol/L and 33.6 mmol/L glucoseconcentrations for 24 h, 48 h, 72 h respectively. The trypan-blue exclusion test was used to study the influence of D-glucose on cell survial.Theexpression of intercellular adhesion molecule-1(ICAM-1), vascular cell adhesion molecule-1(VCAM-1) were measured by flowcytometry.Results When D-glucose concentrations rose trypan-blue excluded cells were decreased. HUVEC exposed to a high glucose concentration (33.6 mmol/L) showed a 1.22-fold increase and a 1.27-fold increase in cell surface expression of ICAM-1 after 48 h and 72 h exposure compared with those cultured in medium with a low glucose concentration( 5.6 mmol/L).Conclusion High concentration of glucose can arrest the proliferative response and show that even a short-term exposure of endothelial cells (ECs) to high glucose concentration leads to their activation associated with increased expression of adhesion molecules such as ICAM-1 and that this effect may appears more significantly along with time of exposuring high concentration of glucose.

关 键 词：高浓度葡萄糖 黏附分子表达 细胞活力 血管细胞黏附分子 ICAM-1表达 VCAM-1 脐静脉内皮细胞 白细胞黏附分子 血管内皮细胞系 细胞间黏附分子 糖尿病血管病变 流式细胞仪检测 培养 慢性并发症 糖尿病患者 早期发病 高糖诱导 

分 类 号：R587.2[医药卫生—内分泌] R743.31[医药卫生—内科学]