高效液相色谱法测定蒙药益智温肾十味丸中的胡椒碱  

DETERMINATION OF PIPERINE IN MONGOLIAN MEDICINE YIZHIWENSHENSHIWEI PILLS BY HPLC

作　　者：许良[1] 席海山[1] 张学富[1] 毕开顺[2] 

机构地区：[1]内蒙古民族大学分析测试中心,通辽028042 [2]沈阳药科大学药学院,沈阳110016

出　　处：《化学分析计量》2005年第3期23-24,共2页Chemical Analysis And Meterage

摘　　要：用高效液相色谱法测定蒙药益智温肾十味丸中胡椒碱的含量。色谱流动相为甲醇-水(体积比为74∶26),流速为0.8mL/min,检测波长为343nm。胡椒碱的质量在0.24～1.2μg内与色谱峰面积有良好的线性关系,r=0.9998。胡椒碱的平均加标回收率为98.7%,测定结果的相对标准偏差为0.93%。该法可作为益智温肾十味丸的含量测定方法。Determination of the content of piperine in Mongolian medicine Yizhiwenshenshiwei Pills was carried out. The mobile phase was methyl alcohol-water (74∶26) with the flow rate of 0.8 mL/min, the wavelength of the detector was set at 343 nm. The linear range was 0.24~1.2 μg, and the correlation coefficient was 0.9998.The average recovery and relative deviation were 98.7% and 0.93% respectively. The method could be used to control the content of Yizhiwenshenshiwei Pills.

关 键 词：高效液相色谱法 胡椒碱 益智 蒙药 肾 相对标准偏差 含量测定方法 色谱峰面积 加标回收率 检测波长 线性关系 测定结果 体积比 醇-水 流动相 平均 

分 类 号：O657.72[理学—分析化学] TS264.3[理学—化学]