离子色谱法测定尿液中的C_2O_4^(2-)、Ca^(2+)、Mg^(2+)  

DETERMINATION OF C_2O_4 ^(2-), Ca^(2+), Mg^(2+) IN URINE BY ION CHROMATOGRAPHY

作　　者：张雪琴 

机构地区：[1]青岛盛瀚色谱技术有限公司,青岛266101

出　　处：《化学分析计量》2005年第3期28-30,共3页Chemical Analysis And Meterage

摘　　要：利用离子色谱法测定尿液中的草酸根、钙、镁离子。测定草酸根时,先在尿液中加入氯化钙使之转化成草酸钙沉淀,分离后用盐酸溶解草酸钙沉淀,然后进行测定。测定钙、镁离子时,在酸性尿液中加入氧化剂过硫酸钾,在微沸状态下氧化尿液中的有机物,从而避免对色谱柱的污染。草酸根、钙、镁离子的回收率分别为108.3%～109.7%、94.9%～100.7%、97.2%～97.4%,相对标准偏差分别为2.4%、2.5%、3.1%。The determination of C_ 2O_4 2-, Ca 2+, Mg 2+ in urine was achieved by ion chromatography. Before C_ 2O_4 2- was determined, calcium chloride was added into the urine sample to form the deposit of calcium oxalate, which dissolved in HCl to make the sample solution. K_ 2S_ 2O_ 8 was used to remove the organic compounds which might pollute the column when Ca 2+ and Mg 2+ were determined. The recoveries of C_2O_4 2-, Ca 2+, Mg 2+ were 108.3%~109.7%, 94.9%~100.7%, 97.2%~97.4% respectively, and the relative standard deviation were 2.4%, 2.5%, 3.1% respectively.

关 键 词：离子色谱法 CA^2+ MG^2+ 尿液 测定 草酸根 镁离子 过硫酸钾 相对标准 草酸钙 氯化钙 酸溶解 氧化剂 有机物 色谱柱 回收率 沉淀 污染 

分 类 号：X832.02[环境科学与工程—环境工程] Q945.172[生物学—植物学]