离子色谱法测定甘草提取物中草酸的含量  

DETERMINATION OF OXALIC ACID IN LIQUORICE EXTRACT BY ION CHROMATOGRAPHY

作　　者：施超欧[1] 栾绍嵘[1] 王洁姮[1] 

机构地区：[1]华东理工大学分析测试中心,上海200237

出　　处：《化学分析计量》2005年第3期38-39,共2页Chemical Analysis And Meterage

摘　　要：建立了用离子色谱测定甘草提取物中残留草酸含量的新方法。选用DionexIonPacAS11-HC阴离子分析柱(250mm×4mmi.d.),淋洗液为18mmol/LNaOH溶液,流速为1.0mL/min,抑制电导检测器检测。草酸色谱峰面积(Y)与浓度(X)在1.004～10.04mg/L内线性关系良好,线性回归方程为Y=0.1939X-0.0326,相关系数r=0.9999,检出限为0.012mg/L。样品回收率为96.8%～98.6%,相对标准偏差为0.78%。该方法简便、快速。A new method for the determination of oxalic acid in liquorice extract by ion chromatography was established. The separation of oxalic acid was obtained by using Dionex Ionpac AS11-HC (250 mm×4 mm i.d.) column and 18 mmol/L NaOH solution as the eluent. In the range of 1.004~10.04 mg/mL, the linear equation was Y=0.1939X-0.0326, the correlation coefficient was 0.9999. The detection limit was 0.012 mg/L. The average recoveries were 96.8%~98.6%, and the relative standard deviation was 0.78%. The method was simple, rapid and accurate.

关 键 词：甘草提取物 离子色谱法 测定 IonPac NAOH溶液 线性回归方程 相对标准偏差 电导检测器 色谱峰面积 草酸含量 线性关系 相关系数 分析柱 阴离子 淋洗液 min 检出限 回收率 

分 类 号：O212.1[理学—概率论与数理统计] TQ464.1[理学—数学]