苎麻叶中绿原酸的分光光度法测定  

Spectrophotometric Determination of Chlorogenic Acid in Leaves of Ramie

作　　者：李勃[1] 瞿龙[1] 施树云[2] 

机构地区：[1]中南大学化学化工学院,湖南长沙410083 [2]浙江大学药学院,浙江杭州310027

出　　处：《广州化学》2005年第2期41-44,50,共5页Guangzhou Chemistry

摘　　要：探讨了采用分光光度法测定苎麻叶中绿原酸的含量的方法.12.5%的醋酸用量为0.1 mL,7%尿素2.0 mL,0.5%亚硝酸钠0.25 mL,3 min后加入5%氢氧化钠0.5 mL,绿原酸与试剂形成鲜红色络合物,通过在510 nm处测定溶液的吸光度确定绿原酸的含量.绿原酸浓度在0.01～0.12 g/L范围内与吸光度值有良好的线性关系,回收率为95%～107%,相对标准偏差(RSD)为2.4%.该方法简便、快速.A method for the spectrophotometric determination of chlorogenic acid in leaves of ramie has been established. The additive amount of acetic acid(12.5%)is 0.1 mL, urea(7%)and sodium nitrite(0.5%)is 2 mL and 0.25 mL respectively. Then 0.5 mL sodium hydroxide(0.5%) was added 3 minutes later. At that time, the red complex was formed, and then chlorogenic acid was determined by spectrophotometer at 510 nm. The linear range is 0.01 ~ 0.12 g/L with a correlation coefficient of 0.9998. The recovery and RSD are 95% ~ 107% and 2.4% respectively. This method is simple and rapid.

关 键 词：苎麻叶 绿原酸 分光光度法 

分 类 号：O657.32[理学—分析化学]