数字化色谱指纹图谱用于连翘的质量控制  

Application of chromatograph-digitized fingerprint in quality assessment of Forsythia Suspensa(thunb.) Vahl

作　　者：曾志[1] 张艳萍[1] 李核[1] 陶敬奇[1] 杨挺[1] 

机构地区：[1]华南师范大学化学系分析测试中心,广东广州510631

出　　处：《中成药》2005年第5期501-505,共5页Chinese Traditional Patent Medicine

基　　金：广东省科技计划项目重大专项(No.A301020101);广东省自然科学基金项目(No. 011442);广东省教育厅高校自然科学研究项目(No. 0124);广东省中医药局科研项目(No. 403047;401039)资助

摘　　要：目的:建立连翘液相色谱指纹图谱,为连翘在中药以及中药制剂的合理利用提供依据。方法:优选萃取条件,采用液相色谱法对不同产地的连翘进行指纹图谱研究。液相色谱条件:InertsilODS柱, 5 的乙腈水溶液与2 的醋酸水溶液作梯度洗脱,检测波长为270nm。结果:实验方法的重现性、样品的稳定性和仪器的精密度均获得良好的结果。结论:指纹图谱法简便,可靠、准确,可用于区分不同产地的连翘。AIM: To establish an effective fingerprint of Forsythia Suspensa , to be applied to identification. METHODS: A gradient separated method was applied with inertsil ODS 3 and with eluent water (5% acetonitrile) water (2% acetic acid). Detection wavelength was set at 290 nm. RESULTS: In comparison with fingerprints of 13 samples of Fructus Forsythiae, this method had high sensitivity,good reproducibility and good stability. CONCLUSION: This method can be applied in identification of Fructus Forsythiae that come from different areas and different sources.

关 键 词：连翘 指纹图谱 高效液相色谱 质量控制 

分 类 号：R284.1[医药卫生—中药学]