蒲黄的黄酮类成分提取工艺研究  

Extraction process of the total flavonoids of Pollen typhae

作　　者：傅大煦[1] 王玮[2] 程小卫[2] 陈家宽[2] 周铜水[2] 

机构地区：[1]上海新药研究开发中心,上海201203 [2]复旦大学生命科学学院,上海200433

出　　处：《中成药》2005年第5期517-520,共4页Chinese Traditional Patent Medicine

摘　　要：目的:优选蒲黄总黄酮的提取工艺条件。方法:以异鼠李素3 0 新陈皮糖苷和香蒲新苷含量为指标,应用L27 (313 )正交试验优化蒲黄总黄酮的提取方法。结果:乙醇浓度、提取时间、提取次数对提取效率有显著性影响。结论:最佳提取工艺为用14倍生药量的40 乙醇提取两次,每次为3h。AIM: To optimize the extraction process of the total flavonoids of Pollen typhae. METHODS: The optimum extraction process was selected with the orthogonal design (L 27 (3 13 )), using the contents of typhaneoside and isorhamnetin 3 O neohesperidoside as the evaluating criteria. RESULTS: The significant effects of alcohol concentration, extraction time and extraction times on the extraction of the total flavonoids of Pollen typhae were discovered. CONCLUSION: The optimum extraction process was as follows: Pollen typhae was extracted with 40% alcohol for two times, each with the solvent volume 14 times of the weight of the raw materials and extraction time for 3 h.

关 键 词：蒲黄 总黄酮 正交试验 提取工艺 

分 类 号：R284.2[医药卫生—中药学]