荧光定量聚合酶链反应检测儿童病毒性脑炎病原体的研究  

The research of fluorescence quantitative polymerase chain reaction (FQ-PCR) method to test children virus encephalitis.

作　　者：高文娟[1] 颜世军[1] 王云霞[1] 王杰英[1] 秦维娜[1] 范彬[1] 任瑞萍[1] 杨清[1] 

机构地区：[1]保定市儿童医院,071000

出　　处：《中国实用儿科杂志》2005年第5期302-304,共3页Chinese Journal of Practical Pediatrics

基　　金：河北省保定市自然科学基金项目(项目编号:03F012)

摘　　要：目的探讨荧光定量聚合酶链反应(FQ PCR)检测儿童病毒性脑炎脑脊液(CSF)中的病原体的价值。方法应用定性聚合酶链反应(PCR)对2002年1月至2004年10月保定市儿童医院收治的病毒性脑炎患儿78例的CSF标本筛选,检测最常见的引起病毒性脑炎的6种病毒,对单纯疱疹病毒(HSV),柯萨奇病毒(CV),埃可病毒(EV)阳性者做FQ PCR检测,其中HSV分别在3个时点(治疗前、治疗中、出院时)检测DNA拷贝数。结果定性聚合酶链反应阳性标本FQ PC检测100%阳性,HSV DNA拷贝数在3个时点有统计学差异,病毒核酸拷贝数与临床表现一致。结论FQ PCR可作为儿童病毒性脑炎早期诊断的有效方法之一,动态观察HSV DNA含量有助于指导治疗。Objective To discuss the value of using FQ-PCR to check pathogen in children viral encephalitis.Methods From Janurary,2002 to October,2004,PCR was used to select the children of viral encephalitis and check the six kinds of common virus which cause viral encephalitis and then we used FQ-PCR to check the children with HSV(+),CV(+)EV(+) while checking the copied number of HSVE-DNA before,during and after treatment.Results It was 100% that FQ-PCR checked the sample of PCR (+).The number of HSV-DNA was different at three time points. The copied number of virus DNA was consistent with clinical symptom.Conclusion FQ-PCR is one of the effective methods to diagnose the children's viral encephalitis.Observing the number of HSV-DNA is beneficial for instructing,treating and estimating the development and future disease.

关 键 词：儿童 病毒性脑炎 病原体 荧光定量聚合酶链反应 PCR 

分 类 号：R72[医药卫生—儿科]