催化系统对聚丙烯酰胺凝胶聚合的影响  

Impact of catalyst on agglomeration of polyacrylamide

作　　者：赵春澎[1] 时岚[2] 孙斐[3] 许兵红[3] 谷兆侠[1] 赵丁奎[1] 

机构地区：[1]新乡医学院生物化学与分子生物学教研室,河南新乡453003 [2]新乡医学院基础医学院科教科 [3]新乡医学院寄生虫学教研室

出　　处：《新乡医学院学报》2005年第3期195-197,共3页Journal of Xinxiang Medical University

基　　金：河南省医学科技创新人才工程项目(NO.200350)

摘　　要：目的探讨不同的催化系统对不同酸碱条件下聚丙烯酰胺凝胶电泳(PAGE)凝胶聚合的影响。方法采用不同剂量的过硫酸铵(AP)四甲基乙二胺(TEMED)催化系统组合和过硫酸铵(AP)硫酸亚铁(FeSO4)-抗坏血酸(VC)催化系统组合,进行碱性PAGE和酸性PAGE,观察各组凝胶聚合所需时间。结果AP-TEMED在碱性电泳中应用,0.05%～0.1%的AP和0.1%的TEMED用量,凝胶聚合在0.5h内完成;在酸性电泳中应用时,0.2%的AP和0.3%～0.4%的TEMED用量,凝胶聚合在2h内完成。AP-VC-FeSO4催化系统应用于酸性电泳时设计的10组用量,48h均未发现凝胶形成。结论AP-TEMED催化系统在碱性PAGE中的效果好于酸性PAGE。在碱性电泳中,以0.05%～0.1%的AP和0.1%的TEMED为宜;在酸性电泳中,以0.2%的AP和0.3%～0.4%的TEMED为宜。Objective Both catalyst system,ammonium persulfate and tetramethyl ethylene diamine(AP-TEMED),and ammonium persulfate and ironsulfate and vitamin C(AP-VC-FeSO_4),were respectively used with different doses in polyacrylamide gel electrophoresis(PAGE).The acidic PAGE and alkaline PAGE were respectively performed to observe the time of formation of gels in different group.Methods Different catalysts at different doses were used in polyacrylamide gel electrophoresis(PAGE),and after the gels formed,the time were recorded and compared.Results It was found that the gel process finished in 0.5 h with 0.05%～0.1% of AP and 0.1% of TEMED in basic electrophoresis,and in 2 h with 0.2% of AP and 0.3%～0.4% of TEMED in acidic electrophoresis.The gel process didn't form even after 48 h in those 10 groups of the acidic electrophoresis with AP-VC-FeSO_4 at different doses.Conclusion The effect of AP-TEMED in basic electrophoresis is better than that in acidic electrophoresis 0.05%～0.1% of AP and 0.1% of TEMED should be adopted in basic electrophoresis,and 0.2% of AP and TEMED of 0.3%～0.4% should be adopted in acidic electrophoresis.

关 键 词：聚丙烯酰胺凝胶电泳 催化系统 硫酸亚铁 过硫酸胺 四甲基乙二胺 

分 类 号：Q5-33[生物学—生物化学]