HBsAg与HBsAb中和比的研究及应用探讨  

Studies on Neutralizing Ratio Between HBsAg and HBsAb and its Potential Usage in Clinical Application

作　　者：滕龙[1] 

机构地区：[1]浙江省温州市第二人民医院,325000

出　　处：《医学研究通讯》2005年第6期17-20,共4页Bulletin of Medical Research

基　　金：温州市科技发展计划项目(S2002A105)

摘　　要：目的明确 HBsAb 与 HBsAg 中和作用不同时间两者之间的消耗比,为 HBsAg/HBsAb 确认试验的标准化奠定基础。方法将卫生部临床检验中心提供的质控血清用电化学发光法测定;并将5ng/ml HBsAg 与30mIU/mlHBsAB 质控血清不同比例37℃作用不同时间后,用电化学发光法测定 HBsAg、HBsAb 浓度的变化;留取日常工作中酶联免疫吸附试验测定病人血清,结果为 HBsAg(+)、HBsAB(-)、HBeAg(-)、HBeAb(+)、HBcAb(+)的标本30份;结果为 HBsAg(-)HBsAB(+)、HBeAg(-)、HBeAb(-)、HBcAb(-)的标本30份,将两种血清不同比例混合37℃作用不同时间用 ELISA、电化学发光法测定 HBsAg、HBsAb 浓度的变化。结果 5ng/ml、2ng/ml、0.5ng/ml HBsAg、30mIU/ml HBsAb 质控血清电化学发光法测定结果分别为116.2 COI、42.43 COI、14.02 COI、62.54 mIU/ml;ELISA 的中和比,以 HBsAg 完全被 HBsAb 中和计算即有223/774=0.283 ng HBsAg/mIU HBsAb,如果以 HBsAb 完全被 HBsAg 中和计算即有570/757=0.753 ng HBsAg/mIU HBsAb。而 ECLIA 的中和比,以 HBsAg 完全被 HBsAb 中和计算即有2304/1664=1.38 COI HBsAg/mIU HBsAb,如果以 HBsAb 完全被 HBsAg 中和计算即有13106/1577=8.31COIHBsAg/mIU HBsAb。5ng/ml HBsAg 与30mIU/mlHBsAB 质控血清37℃水浴作用1h 中和比在3.00～4.25COI HBsAg/mIU HBsAb 之间;2h 中和比在2.31～3.47COI HBsAg/mIU HBsAb 之间;24h 中和比在2.03～2.43COI HBsAg/mIUHBsAb 之间。结论 ELISA 完全中和比在0.283～0.753 ng HBsAg/mIU HBsAb;ECLIA 完全中和比在1.38～8.31 COI HBsAg/mIUHBsAb。Objective In order to collect experimental data for setting standardization of the determining test of HBsAg/HBsAb,we set the experiments to examine the expending ratio of the neutralizing effect between HBsAb and HBsAg at different time length.Methods A set of sam- ples with different concentrations of HBsAg along with the control serum with 30 mIU/ml HBsAB from National Center for Clinical Laboratory affil- iated to ministry of public health were detected by the method of electrochemical luminescence.Concentration variations of HBsAg and HBsAb in the samples made of the same quantity of HBsAg neutralized by different quantifies of the control serum at 37℃ for a serial periods of time,were analyzed as well.One group of 30 serum samples with HBsAg(+),HBsAb(-),HBeAg(-),HBeAb(+)and HBcAb(+)and the oth- er group of 30 serum samples with HBsAg(-),HBsAb(+),HBeAg(-),HBeAb(-)and HBcAb(-)according to the results of ELISA were collected from routine clinical cases.Two samples,one from each group,were mixed with different quantity ratios and the neutraliz- ing reactions were processed at 37℃ for a serial period of time.Concentration variations of HBsAg and HBsAb in these samples were detected by the methods of ELISA and the electrochemical luminescence.Results By the method of the electrochemical luminescence,results of HBsAg with the concentrations of 5 ng/ml,2 ng/ml and 0.5 ng/ml were 116.2 COI,42.43 COI and 14.02 COI,respectively,and the result of the control serum was 62.54 mIU/ml.Relayed on the basis of that HBsAg is totally neutralized by HBsAb,the neutralizing ratio by ELISA was 0.283 ng HB- sAg/mIU HBsAb(9127/774),while relayed on the basis of that HBsAb is totally neutralized by HBsAg,the neutralizing ratio by ELISA was 0.753 ng HBsAg/mIU HBsAb(2304/1664).In other hand,the neutralizing ratio by ECLIA was 1.38 COI HBsAg/mIU HBsAb(2304/1664) for the former and 8.31 COI HBsAg/mIU HBsAb(13106/1577)for the later.The neutralizing ratios of 5 ng/ml HBsAg neutralized with 30 mIU/ ml HBsAB control serum at 37 ℃ for 1

关 键 词：HBSAB 电化学发光法 酶联免疫吸附试验 HBSAG(+) ELISA HBSAB ECLIA 质控血清 不同时间 临床检验中心 不同比例 COI HBSAG 37℃水浴 中和作用 确认试验 病人血清 日常工作 测定结果 ml 标准化 卫生部 24h 浓度 标本 

分 类 号：R512.62[医药卫生—内科学] R542.22[医药卫生—临床医学]