铁氰化钾-鲁米诺体系后化学发光行为的研究-流动注射后化学发光法测定可待因  

Investigation on the post chemiluminescence behavior of codeine in luminol-potassium ferricyanide reaction

作　　者：冯娜[1] 何云华[1] 杜建修[1] 吕九如[1] 

机构地区：[1]陕西师范大学化学与材料科学学院,西安710062

出　　处：《分析试验室》2005年第5期1-3,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金资助项目(20275023);陕西省自然科学基础研究计划项目(2002B12)

摘　　要：发现了可待因在铁氰化钾鲁米诺化学发光反应体系中的后化学发光反应。优化了反应条件,建立了一种利用后化学发光反应测定可待因的流动注射化学发光新方法。方法的检出限为3×10-8g mL,相对标准偏差为1.9%(1.0×10-6g mL可待因,n=11),线性范围为8.0×10-8～1.0×10-5g mL。此法已用于可待因片剂中可待因的测定,结果与药典方法测定值一致。A new chemiluminescence (CL) reaction called post-chemiluminescence reaction was found when codeine was injected into the reaction mixture of alkaline luminol and potassium ferricyanide. The experimental conditions of the CL reactions were optimized. A new chemiluminesence method for the determination of codeine with flow injection technique was developed. The detection limit is 3×10^(-8) g/mL, the relative standard deviation is 1.9% (1.0×10^(-6) g/mL codeine, n=11), the linear range is 8.0×10^(-8)～1.0×10^(-5) (g/mL). The method has been successfully applied to the determination of codeine in codeine tablets and the results are in good agreement with those obtained by pharmacopoeia method.

关 键 词：可待因 后化学发光反应 流动注射 鲁米诺 铁氰化钾 

分 类 号：O557.3[理学—热学与物质分子运动论]