虎杖提取物中白藜芦醇的荧光分析研究  

Study on the fluorometry for determining the content of resveratrol in extract of polgonum cuspidatum siab at zncc

作　　者：张敏[1] 曹庸[1] 于华忠[1] 彭密军[1] 龚竹琼[1] 李贵[1] 

机构地区：[1]吉首大学林产化工工程重点实验室,张家界427000

出　　处：《分析试验室》2005年第5期15-18,共4页Chinese Journal of Analysis Laboratory

基　　金：湖南省科技厅重点科技攻关资助项目(03JZY3020)

摘　　要：建立了一种荧光检测虎杖提取物中白藜芦醇的新方法,探讨了白藜芦醇荧光检测的最佳条件,其激发波长和发射波长分别是336和380nm。白藜芦醇荧光强度与浓度在0～1.68×10-5mol L的范围内具有良好的线性关系,相对标准偏差为2.39%～4.45%。检出限为8.84×10-10mol L。A new fluorometric method for determining the content of resveratrol in extract of polgonum cuspidatum siab at zncc was developed .The optimal condition of fluorosence determination for resveratrol was established. The excitation and emission wavelengths are 336 and 380 nm. The linear relationship between the fluorosence intensity and the concentration of resveratrol was over the range of 0～～1.68×10^(-5) mol/L, a detection limit was 8.839×10^(-10) mol/L and a relative standard deviation was 2.39～4.45%. The method is simple, rapid and sensitive.

关 键 词：荧光分析法 白藜芦醇 虎杖 

分 类 号：O657.32[理学—分析化学]