次甲基蓝褪色光度法测定脱氧核糖核酸  

Determination of Deoxyribonucleic Acid with Methylene Blue by Spectrophotometry

作　　者：李山[1] 沈春强[1] 闫明娟[1] 

机构地区：[1]长安大学环境科学与工程学院,西安710054

出　　处：《分析试验室》2005年第6期36-38,共3页Chinese Journal of Analysis Laboratory

摘　　要：研究了碱性染料次甲基蓝与脱氧核糖核酸的结合反应。在pH8.0～8.5的三羟甲基氨基甲烷-HCl介质中,次甲基蓝于630nm处有最大吸收,随着脱氧核糖核酸加入量的增加,其在630nm处的吸光度显著下降,下降程度与脱氧核糖核酸的量成正比。据此,建立了测定脱氧核糖核酸的新方法,方法的线性范围为0～7mg/L。该方法可用于合成样品分析。The binding reaction of deoxyribonucleic acid with methylene blue has been studied. In a pH range of 8.0～8.5, the system shows an absorption peak (630 nm), and this peak decreases with the increasing deoxyribonucleic acid concentration. Based on this, a new method for the determination of deoxyribonucleic acid at 630 nm using methylene blue as a labeling agent has been developed, and the linear range of determination is 0～7 mg/L. The method has been applied to the determination of deoxyribonucleic acid in synthetic samples with satisfactory results.

关 键 词：脱氧核糖核酸 次甲基蓝 褪色作用 分光光度法 

分 类 号：O657.32[理学—分析化学]