Construction and analysis of a subtracted cDNA library of Betula platyphylla female inflorescence  

作　　者：WEIJi-cheng YANGChuan-ping WANGChao JIANGJing 

机构地区：[1]ForestryCollege,NortheastForestryUniversity,Harbin150040,P.R.China [2]ForestryCollege,NortheastForestryUniversity,Harbin150040,P.R.China／／MudanjiangTeachersCollege,Mudanjiang157012,PR.China

出　　处：《Journal of Forestry Research》2005年第2期97-100,共4页林业研究（英文版）

基　　金：This project was supported by National Natural Science Foundation of China (39970627)

摘　　要：Female inflorescence of Betula platyphylla was sampled at an interval of eachtwo days to analyze the background of gene expression in floral phase. On the basis of SMARTstrategy, the driver cDNA was obtained from total RNA of the last sample and the tester cDNA wasfrom that of the others by RT-PCR which were subsequently used to construct a subtracted cDNAlibrary. The result of the ESTs (expression sequence tags) blastX showed that the genes in thesubtracted cDNA library could be mainly clustered into 5 groups related to metabolism,transportation and signal transduction, cell cycle, stress response, and regulation. Therelationship between gene expression and development was also discussed.Female inflorescence of Betula platyphylla was sampled at an interval of eachtwo days to analyze the background of gene expression in floral phase. On the basis of SMARTstrategy, the driver cDNA was obtained from total RNA of the last sample and the tester cDNA wasfrom that of the others by RT-PCR which were subsequently used to construct a subtracted cDNAlibrary. The result of the ESTs (expression sequence tags) blastX showed that the genes in thesubtracted cDNA library could be mainly clustered into 5 groups related to metabolism,transportation and signal transduction, cell cycle, stress response, and regulation. Therelationship between gene expression and development was also discussed.

关 键 词：betula platyphylla subtracted cDNA library SMART 

分 类 号：S792.153[农业科学—林木遗传育种]