木霉的空间诱变效应  被引量:9

SPACE MUTAGENIC EFFECT OF Trichoderma Reesei

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作  者:田兴山[1] 张玲华[1] 郭勇[2] 周风珍[1] 黄小光[1] 邝哲师[1] 潘木水[1] 李国立[1] 

机构地区:[1]广东省农科院农业生物技术研究所,广东广州510640 [2]华南理工大学生物工程学院,广东广州510640

出  处:《核农学报》2005年第3期195-197,213,共4页Journal of Nuclear Agricultural Sciences

摘  要:本文对产纤维素酶的木霉菌孢子和斜面培养菌体搭载中国第18颗返回式卫星后,菌落形态和产酶能力的变化进行了研究。实验结果表明,搭载菌株的菌落形态发生变异,菌株的生长周期出现了缩短或延长的现象。对搭载的木霉菌株进行筛选,获得3株产酶能力大幅度提高的优良突变菌株,产酶能力提高了50%以上,且性状遗传稳定。The slant mycelia cultured with or without mutagenic agent diethyl sulfate(DS) and spores of Trichoderma reesei were loaded in the 18^(th) returning satellite. Systematical screening showed that the life cycle and morphology of some strains had changed after space flight. After selection and domestication, 3 mutant strains with high cellulose enzyme activity were isolated. The cellulose enzyme productivities of the mutants were significantly increased more than 50%, and the mutant were generically stable.

关 键 词:空间诱变效应 产酶能力 返回式卫星 斜面培养 霉菌孢子 纤维素酶 菌落形态 形态发生 生长周期 木霉菌株 突变菌株 能力提高 遗传稳定 搭载 大幅度 

分 类 号:Q933[生物学—微生物学]

 

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