重金属对水稻光合作用和同化物输配的影响  

EFFECT OF HEAVY METALS ON THE PHOTOSYNTHESIS AND PHOTOSYNTHATES TRANSFORMATION IN RICE

作　　者：葛才林[1] 骆剑峰[1] 刘冲[1] 殷朝珍[1] 王泽港[1] 马飞[1] 罗时石[1] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室,江苏扬州225009

出　　处：《核农学报》2005年第3期214-218,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金资助项目(30300026)

摘　　要：光合速率测定表明,0.025～1.0mmolL的Cu2+、Cd2+和Hg2+显著抑制水稻叶片的光合速率,且抑制程度随Cu2+、Cd2+和Hg2+浓度的增高而增强。示踪动力学分析表明,0.5mmolL的Cu2+、Cd2+和Hg2+抑制光合产物在叶内不同状态间的转化,影响叶中光合产物的输出,导致叶内小分子糖的累积,使叶片光合速率受糖浓度增高的反馈调节而下降。电泳分析表明,Cu2+、Cd2+和Hg2+抑制叶内淀粉酶同功酶的表达,并进而抑制光合同化物在叶内可输配态和暂贮态之间的转化。The photosynthetic rate in rice leaf was significantly inhibited by 0.025～1.0mmol/L of Cu^(2+), Cd^(2+) and Hg^(2+). With increasing of Cu^(2+), Cd^(2+) and Hg^(2+) concentration, the inhibiting degree of photosynthetic rate was enhanced. Tracer kinetic analysis indicated that, 0.5mmol/L Cu^(2+), Cd^(2+) and Hg^(2+) inhibited the transformation between starch and sugar, disturbed the translocation of photosynthates from leaves. Consequently, sugar accumulated in the leaves, which might induce a feedback-regulation of photosynthetic rate. Using polyacrylamide concentration gradient gel electrophoresis to analyze the activity of amylase isozymes indicateed that, the amylase isozymes expression in rice leaf was inhibited by 0.1～0.5mmol/L Cu^(2+), Cd^(2+) and Hg^(2+), hence, the transformation between starch and sugar was blocked.

关 键 词：光合作用 输配 重金属 CU^2+ HG^2+ CD^2+ 叶片光合速率 淀粉酶同功酶 光合产物 示踪动力学 光合同化物 速率测定 水稻叶片 反馈调节 浓度增高 分析表 抑制 小分子 转化 累积 

分 类 号：S511[农业科学—作物学]