双纸片法检测葡萄球菌中可诱导的克林霉素耐药  

Double disk diffusion method for detection of inducible clindamycin resistance in Staphylococci

作　　者：应春妹[1] 汪雅萍[1] 甘如饴 杨海慧[1] 刘立成[3] 

机构地区：[1]上海第二医科大学附属仁济医检验科,200127 [2]上海市公利医院 [3]上海市疾病预防控制中心

出　　处：《中国抗感染化疗杂志》2005年第3期171-173,共3页Chinese Journal of Infection and Chemotherapy

摘　　要：目的了解我院葡萄球菌对常用抗菌药物的耐药性及可诱导克林霉素耐药发生率。方法KirbyBauer法检测2004年5—7月我院268株葡萄球菌对14种抗菌药物的耐药性,双纸片法检测可诱导克林霉素耐药。结果替考拉宁和万古霉素抗菌活性最强,136株金黄色葡萄球菌中有124株为红霉素耐药,其中115株为持续性克林霉素耐药,7株为可诱导性克林霉素耐药。132株凝固酶阴性葡萄球菌中有106株为红霉素耐药,其中67株为持续性耐药,16株为可诱导性克林霉素耐药。结论临床微生物实验室须加强可诱导克林霉素耐药的检测,以指导临床合理使用抗生素。Objective To investigate the susceptibility of clinical isolates of Staphylococci from Renji Hospital and the prevalence of inducible clindamycin resistance in Staphylococci.Methods Bacterial susceptibility testing was carried out on 268 strains of Staphylococci isolated from Renji Hospital from May to July 2004 using Kirby-Bauer method. Inducible clindamycin resistance was detected by double disk diffusion method.Results Teicoplanin and vancomycin were the most active agents against Staphylococci. Of 124 erythromycin-resistant S.aureus isolates, 115 demonstrated constitutive resistance to clindamycin, while 7 showed inducible resistance. Of 106 erythromycin-resistant CNS isolates, 67 demonstrated constitutive resistance to clindamycin, while 16 had inducible resistance. Conclusions The detection of inducible clindamycin resistance must be stressed in clinical microbiology laboratory to provide good support for rational antibiotic therapy.

关 键 词：葡萄球菌 克林霉素耐药 

分 类 号：R446.5[医药卫生—诊断学]