Hydrodynamic Shear Stress Affects Cell Growth and Metabolite Production by Medicinal Mushroom Ganoderma lucidum  

作　　者：龚海刚 钟建江 

机构地区：[1]State Key Laboratory of Bioreactor EngineeringEast China University of Science and Technology,Shanghai 200237,China

出　　处：《Chinese Journal of Chemical Engineering》2005年第3期426-428,共3页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No. 20076011, No. 20236040 and No. 20225619).

摘　　要：By investigating the shear effect on submerged cultivation of a traditional Chinese medicinal herb Ganoderma lucidum, a relatively high cell concentration of 13.8 g·L-1 by dry mass was obtained in bioreactor at an impeller tip speed (ITS) of 0.51m·s-1. At an ITS of 0.51,1.02 and 1.53m·s-1, a maximal production titer of intracellular polysaccharide was 2.64, 2.20 and 2.28g·L-1 and that of ganoderic acid was 306, 299 and 273g·L-1, respectively. Under these ITSs, the maximal mean projected area of dispersed hyphae was 3.70, 2.54 and 2.13×104μm2, and that of pellets was 0.91, 0.67 and 0.55mm2, respectively. The information obtained is useful for efficient submerged cultivation of mushrooms on a large scale.By investigating the shear effect on submerged cultivation of a traditional Chinese medicinal herb Ganoderma lucidum, a relatively high cell concentration of 13.8 g·L^-1 by dry mass was obtained in bioreactor at an impeller tip speed (ITS) of 0.51m·s^-1. At an ITS of 0.51,1.02 and 1.53m·s^-1, a maximal production titer of intracellular polysaccharide was 2.64, 2.20 and 2.28g·L^-1 and that of ganoderic acid was 306, 299 and 273g·L^-1, respectively. Under these ITSs, the maximal mean projected area of dispersed hypheue was 3.70, 2.54 and 2.13 × 10^4μm^2, and that of pellets was 0.91, 0.67 and 0.55 mm^2, respectively. The information obtained is useful for efficient submerged cultivation of mushrooms on a large scale.

关 键 词：Ganoderma lucidum mushroom cultures shear effect Ganoderma polysaccharide ganoderic acid BIOREACTOR 

分 类 号：S567.3[农业科学—中草药栽培]