高效液相色谱法分析醇醚糖苷化后的混合物  

Analysis of mixture of glucosidation of alcohol ethers by RP-HPLC

作　　者：左安宝[1] 姚晨之[1] 杨秀全[1] 程玉梅[1] 王虎[1] 

机构地区：[1]中国日用化学工业研究院,山西太原030001

出　　处：《应用化工》2005年第5期304-307,共4页Applied Chemical Industry

基　　金：山西省技术创新项目(财2003-052)

摘　　要：对脂肪醇聚氧乙烯(3)醚(AEO3)进行糖苷化,用反相高效液相色谱法对糖苷化后的混合物进行了检测分析。确定了色谱条件:C18Diamonsil,5μm,4.6×250mm色谱柱,乙氰∶N,N 二甲基甲酰胺=1∶4(体积比)为流动相,示差折光检测器。结合用化学法测得的未反应葡萄糖、生成的二糖多糖的质量百分含量,可计算出AEO3糖苷化后混合物中的糖苷化物的质量百分含量。该方法的相对标准偏差小于5%。The glucosidation of aliphatic alcohol (3) ethoxylates(AEO_3) was made and the production mixture was analysed by reverse phase-high performance liquid chromatograph(RP-HPLC). A good condition for RP-HPLC was established: C_(18)Diamonsil, 5 μm,4.6×250 mm chromatographic column,acetonitrile∶N,N-dimethyl formamide=1∶4(volume ratio)as the mobile phase and a differential refractive index detector.The mass ratio of glucosides was figured out with the unreact ratio of glucose and polysaccharides detected by chemical method. The CV is less than 5%.

关 键 词：脂肪醇聚氧乙烯醚 烷基醚多苷 烷基多苷 无水葡萄糖 高效液相色谱法 分析 

分 类 号：O657.72[理学—分析化学]