两种冷季型草坪草愈伤组织诱导研究初报  

Studies on Callus Induction of Two Cool-Season Turfgrass Cultivars

作　　者：段碧华[1] 韩宝平[1] 高遐虹[1] 孙月梅[1] 

机构地区：[1]北京农学院植物科学技术系,北京102206

出　　处：《北京农学院学报》2005年第1期65-67,共3页Journal of Beijing University of Agriculture

基　　金：北京市科技新星计划项目(项目编号:H020821300120)

摘　　要：以草地早熟禾(PoapratensisL.)2品种即超级伊克利(SuperEclipse)、新哥来德(Nuglade)和多年生黑麦草(LoliumperenneL.)1品种即爱神特(Accent)的成熟种子为外植体,研究了不同质量浓度2,4D和6BA对各品种愈伤组织诱导的影响。结果表明:(1)愈伤组织诱导和继代的最佳培养基配方为:草地早熟禾为:MB5+6BA0.5mg/L+2,4D3.0mg/L;多年生黑麦草为:MS+6BA0.5mg/L+2,4D5.0mg/L。(2)愈伤组织质量最好的培养时间为20～40d。The mature seeds of Poa Pratensis.L.cv(Super Eclipse、Nuglade) and Lolium perene(Accent) were used for explants in this test. Effect of the different 2,4-dichlorophenoxyacetic acid(2,4-D) and 6-benzyladenine(6-BA) on callus induction were studied . The result showed that:at First,the best medium about callus induction of Poa.pratensis L. is MB5+6-BA0.5 mg/L+2,4-D 3.0 mg/L and Lolium perene L.is MB5+6-BA0.5 mg/L+2,4-D 5.0 mg/L;Secondly,the best time of callus quality was from twenty to fourty days.

关 键 词：愈伤组织诱导 冷季型草坪草 研究初报 多年生黑麦草 草地早熟禾 培养基配方 成熟种子 质量浓度 培养时间 品种 外植体 6BA 继代 

分 类 号：S567.239[农业科学—中草药栽培] S688.4[农业科学—作物学]