启东鸭肝癌中人HBV变异体DNA的PCR扩增  

PCR AMPLIFICATION OF HUMAN HBV LIKE DNA SEQUENCE IN DUCK HEPATOMA FROM QI-DONG COUNTY OF CHINA

作　　者：张学理[1] 陈莹[1] 万大方[1] 蒋惠秋[1] 顾健人[1] 

机构地区：[1]上海市肿瘤研究所"癌基因与相关基因"国家重点实验室

出　　处：《肿瘤》1994年第4期190-192,共3页Tumor

摘　　要：自在启东鸭肝癌中证实存在人ＨＢＶ样ＤＮＡ后［１，２］，进一步的实验证明人ＨＢＶ样ＤＮＡ在启东鸭肝癌中带有普遍性和特征性［３］。本实验在上述工作的基础上取４号、８号、９号三个鸭肝癌样品，对ＨＢＶ中９７２－１２７２片段进行ＰＣＲ扩增，经Ｓｏｕｔｈｅｒｎ杂交分析证实有阳性条带，对４号样品ＰＣＲ产物部分核苷酸顺序分析，观察到与ａｄｗ、ａｄｗ２、ａｄｒ、ａｙｗ和ａｙｒ分别有８１．３％、８２．１％、７７．６％、８０．０％和７９．４％的同源性。进一步证明启东鸭肝癌中确实存在人ＨＢＶ变异体ＤＮＡ。The presence of HBV-like DNA sequence in duck hepatoma collected from Qi-Dong County of China was reported in 1988.Here, we amplified three duck hepatoma DNA samples with PCR method (Human HBV nucleotide 972-1272 fragment).After southern blot hybridization,the positive bands were observed.Analysing a part of the DNA sequence of a PCR product (pDCHBV),we observed that it had 81.3% homology to adw,82.1% homology to adw2,77.6% homology to adr,80.0% homology to ayw and 79.4% homology to ayr subtype of HBV.These results further confirm that human HBV mutant DNA sequence occurred in duck hepatoma samples from Qi-Dong county of CHina.

关 键 词：鸭肝癌 乙型肝炎病毒 DNA 

分 类 号：S858.327.7[农业科学—临床兽医学]