胺碘酮对兔缺血再灌注纤溶活性、内皮血管活性物质的影响  

The effect of amiodarone on fibrinolytic activity and vasoactive mediators in ischemic reperfusion in rabbits

作　　者：刘恒亮[1] 陈奇 刘灵芝[1] 吴莉华[1] 康运凯[1] 张文莲[1] 

机构地区：[1]河南省郑州市第五人民医院,河南郑州450003 [2]郑州澍青医学院,河南郑州450003

出　　处：《中国现代应用药学》2005年第3期199-202,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：郑州市自然科学基金项目(NO:20001205)

摘　　要：目的探讨兔缺血再灌注纤溶活性、内皮血管活性物质的变化及胺碘酮的影响。方法新西兰大白兔60只,随机分为5组,每组12只,Ⅰ组:假手术组,Ⅱ组:急性心肌梗死(acutemyocardialinfarction,AMI)组,Ⅲ组:缺血再灌注(ischemicreperfusion,IR)组,Ⅳ组:IR+利多卡因组,Ⅴ组:IR+胺碘酮组;各组(除Ⅰ组外)分别结扎冠状动脉左室支中点,缺血60min,再灌注240min(Ⅰ,Ⅱ组不进行再灌注),分别取结扎前、再灌注前、再灌注240min血测定内皮素(endochelin,ET)、一氧化氮(nitricoxideNO)浓度和组织型纤溶酶原激活剂(tissuetypeplasminogenactivator,tPA)、纤溶酶原激活剂抑制物(plasminogenactivatorinhibitorPAI)活性。结果冠状动脉结扎后,血浆ET,NO浓度和PAI活性显著高于结扎前(P<0.01),tPA活性显著低于结扎前(P<0.01),再灌注后,血浆ET,NO浓度和PAI活性进一步升高,tPA活性进一步下降,与再灌注前对比均有显著性差异(P<0.01)。再灌注后,与IR组对比,胺碘酮能显著降低血PAI活性和ET浓度(P<0.01);利多卡因组无显著变化。结论胺碘酮可抑制缺血再灌注过程中PAI活性、抑制内皮细胞释放ET的有益作用。OBJECTIVE To explore the changes of fibrinolytic activity and vasoactive mediators in ischemic reperfusion in rabbits and the effect of intervention of amiodarone and lidocaine.METHODS Sixty New Zealand white rabbits were randomly assigned to five groups, twelve for each. Group Ⅰ: sham group, Group Ⅱ: acute myocardial infarction(AMI) group, Group Ⅲ: ischemic reperfusion(IR)group, Group Ⅳ: IR+lidocaine group, Group Ⅴ: IR+amiodarone group. The middle point of left ventricular coronary artery was ligated for 60 minutes(except group I), After that, reperfusion for 240 minutes(except groupⅠandⅡ). Before ligation, before and 240 minnutes after reperfusion, blood was collected for measuring the plasma concentration of endochelin (ET), nitric oxide (NO) and the plasma activity of tissue-type plasminogen activator(t-PA), plasminogen activator inhibitor(PAI) respectively.RESULTS After ligation, the plasma concentration of ET,NO and the plasma activity of PAI were significantly higher than those before ligation( P <0.01), but the plasma activity of t-Pa were remarkably decreased than those before ligation( P <0.01). After reperfusion, plasma concentration of ET, NO and plasma activity of PAI were significantly higher than those before reperfusion( P <0.01),conversely, the plasma activity of t-Pa were remarkably decreased than those before reperfusion( P <0.01). After reperfusion, the plasma concentration of ET and plasma activity of PAI in amiodarone group were significantly decrease than those in IR group ( P < 0.01 ). Lidocaine had no effect above. CONCLUSION Amiodarone can inhibit PAI activity, decrease release of ET in ischemic reperfusion in rabbits.

关 键 词：缺血再灌注 纤溶活性 内皮素 一氧化氮 胺碘酮 

分 类 号：R972.2[医药卫生—药品]