乙型肝炎病毒基因组前-前-S和前-X区基因分布的研究  

Distribution of pre-pre-S and pre-X sequences in hepatitis B virus genome of different genotypes

作　　者：段学章[1] 庄辉[1] 杜珩[1] 方钟燎[1] 

机构地区：[1]北京大学医学部病原生物学系,100083

出　　处：《中华微生物学和免疫学杂志》2005年第5期348-350,共3页Chinese Journal of Microbiology and Immunology

基　　金：北京市科委课题 (No .H0 2 0 92 0 0 2 0 190 )

摘　　要：目的　研究不同基因型乙型肝炎病毒(HBV)前前S和前 X区核苷酸和氨基酸序列分布。方法　应用DNAstar软件中的MegAlign程序分析GenBank登记的35株和3株由本室测定的不同基因型HBV全序列中前- 前-S和前 X区基因分布情况,并用Blast软件对GenBank中5 98株HBV全基因组序列进行前- 前- S和前X区的核苷酸和氨基酸相似性分析。结果　除D基因型外,在各型HBV基因组中均存在前- 前-S区核苷酸序列,但仅在C、F和H型HBV基因组中存在前 -前- S区开放读码框架(ORF)。但前X区核苷酸序列及其ORF仅见于C基因型HBV。在GenBank登记的5 98株不同基因型HBV中,与推导的前-前- S区相似氨基酸序列有36株,而与推导的前X区相似的氨基酸序列有4 7株。结论　前 -前- S区序列存在于除D基因型以外的所有HBV基因型,而前 X区基因仅存在于C基因型HBV基因组中。Objective To investigate the distribution of p re -pre-S and pre-X sequences in hepatitis B virus genome of different genotypes . Methods The distribution of pre-pre-S and pre-X sequ ences was analyzed in 38 hepatitis B virus (HBV) complete sequences of different genotypes(35 from GenBank and 3 from our laboratory) using MegAlign program fro m DNAstar software. The Blast software was used to find the sequences of nucleot ide acids and deduced amino acids similar to pre-pre-S and pre-X region in 59 8 HBV strains from GenBank. ResultsSequences of pre-pre -S region were present in all genotypes of HBV except genotype D. Pre-pre-S s equences, identified as an open reading frame (ORF), were only found in HBV geno me of genotype C, E and H. Sequences of pre-X as well as its ORF were only foun d in HBV genome of genotype C. Of 598 HBV strains of different genotypes from Ge nBank, 36 amino acid sequences shared high similarity with pre-pre-S sequence, and 47 amino acid sequences had high similarity with pre-X analyzed by Blast s oftware. Conclusion The pre-pre-S sequences are present in all HBV genotypes except genotype D, while pre-X sequences are found only i n HBV genotype C.

关 键 词：乙型肝炎病毒 基因组 前-前-S 前-X区 基因分布 

分 类 号：R373.2[医药卫生—病原生物学]