FasL对CD28基因启动子活性的抑制作用  

Inhibitory effect of FasL on the promoter activity of CD28

作　　者：马世彬[1] 徐丽荣[2] 宋文刚[2] 何维[1] 

机构地区：[1]中国医学科学院基础医学研究所免疫室,北京100005 [2]泰山医学院免疫学教研室

出　　处：《中华微生物学和免疫学杂志》2005年第5期393-396,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家 973资助项目 (2 0 0 1CB5 10 0 0 9) ;日本老化制御研究所资助项目

摘　　要：目的　探讨FasL对CD2 8基因启动子活性的影响,为研究凋亡信号在免疫衰老中的作用提供资料。方法　采用MTT法和annexinⅤ- FITC染色测定细胞凋亡,Northernblot检测CD2 8mRNA的表达水平,构建CD2 8基因上游非编码区序列的报告基因载体,并瞬时转染JurkatE6 - 1细胞后,用Luciferase检测报告基因启动子的活性。结果　FasL在JurkatE6 -1细胞介导的凋亡可使CD2 8mRNA水平降低。CD2 8启动子的有效序列在上游- 4 0 0bp的范围内,并且FasL介导的凋亡信号可明显降低CD2 8启动子活性。结论　FasL介导的凋亡信号是CD2 8基因启动子活性降低的重要原因。Objective To study the effect of FasL on the p ro moter activity of CD28, and to provide evidence for the effect of apoptotic sign al in immunosenescence. Methods Cell apoptosis was detect ed by MTT and annexin Ⅴ-FITC. The mRNA level of CD28 is measured by Northern b lot. Report vectors were constructed by routine molecular biological methods and Luciferase assay was performed to detect the promoter activity of sequences of CD28 upstream gene after instant transinfection. Results We have proved that the level of CD28 mRNA is reduced because of apoptotic signa l triggered by FasL in Jurkat E6-1 cells. The effective promoter was located in -400 bp in the sequences of CD28 upstream gene. The promoter activity was obvio usly depressed by apoptotic signal trigged by FasL. Conclusion Apoptotic signal trigged by FasL was a key factor in the inhibition of promoter activity.

关 键 词：FASL CD28基因 启动子活性 抑制作用 凋亡信号 

分 类 号：R392[医药卫生—免疫学]