糖尿病病程对真皮成纤维细胞生物学行为的影响  

Effects of Different Courses of Disease on the Biological Behavior of Dermal Fibroblasts in Diabetic Rats

作　　者：王敏骏[1] 陆树良[1] 青春[1] 花兰女[1] 史桂英[1] 盛昭园[1] 廖镇江[1] 

机构地区：[1]上海第二医科大学瑞金医院灼伤整形科上海市烧伤研究所,上海200025

出　　处：《上海第二医科大学学报》2005年第5期459-462,466,共5页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家重点基础研究发展计划(973计划)(G1999054205)资助项目.

摘　　要：目的探讨病程对糖尿病皮肤和真皮成纤维细胞(FB)生物学行为的影响。方法通过制备不同病程糖尿病模型,对皮肤中的组织生化和真皮FB的生物学行为进行检测。结果短程糖尿病皮肤厚度变薄,糖含量升高,且病程至8周有晚期糖基化终末产物(AGEs)蓄积,真皮S期细胞比例升高和单位面积皮肤羟脯氨酸含量减少。高糖和AGEHSA导致真皮FB生长抑制和凋亡,呈剂量依赖性。结论糖尿病皮肤随着病程延长,病理生理异常会不断加重;糖尿病真皮FB的生物学改变与皮肤高糖和AGEs蓄积存在直接关系。Objective To study the effects of different courses of disease on biological behavior of dermal fibroblasts in diabetic rats. Methods Diabetic animal models with different courses of disease (short/long-term group) were prepared to study the changes of histology, biochemistry and the biological behavior of dermal fibroblasts in diabetic intact skin. Results The thickness of skin layer and the contents of glucose were reduced in short-term group. In long-term group, the contents of advanced glycation end products (AGEs) in diabetic skin and the percentage of S stag in the dermal cells were higher than those of controls. The contents of hydroxyproline in the same area were reduced obviously in long-term group with the morphological characteristic changes including the degenerated collagen fibers with focal chronic inflammatory cells infiltration. Proliferation of dermal fibroblasts derived from each group was significantly lower than control group when exposed to the high concentrations of AGE-HSA. The apoptotic rates of fibroblasts received from three groups were significantly higher than control group when cultured with AGE-HSA. The proliferation and apoptotic rate of fibroblasts showed a dose dependent effect with AGE-HSA. Conclusion It is found that there is a set of abnormal pathological changes in the diabetic skin. When the course of diabetes is prolonged, abnormality in the diabetic skin will aggravate continuously. The high contents of dermal glucose and AGEs in the diabetic rat skin show the inhibiting effects on the biological behaviors of dermal fibroblasts, this might be one of the most important mechanisms in the pathogenesis of impaired-wound healing.

关 键 词：细胞生物学行为 糖尿病病程 晚期糖基化终末产物 真皮成纤维细胞 S期细胞比例 糖尿病模型 剂量依赖性 不同病程 皮肤厚度 生长抑制 病程延长 生理异常 AGEs FB 糖含量 酸含量 蓄积 高糖 

分 类 号：R587.2[医药卫生—内分泌]