伤灶局部引流和亚低温联合治疗创伤性脑损伤的实验研究  

Treatment Effects of Focus Hypothermia and Drainage on Traumatic Brain Injury of Sprague-dawley Rats

作　　者：罗超[1] 刘运生[1] 赵贤军[1] 徐立新[1] 李玉斌[1] 

机构地区：[1]中南大学湘雅医院神经外科,湖南长沙410008

出　　处：《医学临床研究》2005年第5期643-646,共4页Journal of Clinical Research

摘　　要：【目的】研究伤灶局部引流、伤灶局部亚低温联合治疗对SD大鼠创伤性脑损伤后脑水含量、Na+含量、血脑屏障变化、脑组织自由基变化的影响,以探讨伤灶局部引流、伤灶局部亚低温对创伤性脑损伤的治疗作用及可能机制。【方法】采用自由落体打击装置造成大鼠创伤性脑损伤,分别在伤后30 min开始实施伤灶局部引流、25℃循环水对伤灶脑组织进行局部降温至(31.5±0.5) ℃,而对假脑外伤组及脑外伤模型组不降温,各组在相应时间点断头处死;采用干湿重法测伤灶脑组织含水量,原子吸收火焰分光光度法测Na+ 含量,采用黄嘌呤氧化酶法检测伤灶周围脑组织匀浆中超氧化物歧化酶(T SOD)含量,硫代巴比妥酸法测定丙二醛(MDA)的含量,双缩脲法测定组织蛋白,伊文思蓝(EB)检测血脑屏障开放情况。【结果】①伤灶局部引流、伤灶局部亚低温联合治疗组含水量低于脑外伤模型组(P<0.05)。②钠离子变化与含水量变化一致,伤灶局部引流、伤灶局部亚低温联合治疗组低于脑外伤模型组(P<0.05)。③MDA含量,联合治疗组低于脑外伤模型组(P<0.05),而T SOD含量联合治疗组高于脑外伤模型组(P<0.05)。④联合治疗组脑组织EB含量低于模型组(P<0.05)。【结论】伤灶局部引流联合伤灶局部亚低温对创伤性脑损伤有治疗作用。这种治疗效果可能是通过直接引流出?ObjectiveTo investigate the effect of focus hypothermia and drainage on traumatic brain injury of Sprague-dawley rats and its mechanism.Feeney's model was used to produce traumatic brain injury in Sprague-dawley rats. After half an hour, the united group of focus hypothermia and drainage was received focus brain hypothermia with 25℃ water except for model group and sham operation group. The injured rats were put to death according to the corresponding time.Brain water content of the united group was markedly less than that of model group (P<0.05). The concentration of brain sodium coincided with that of water content (P<0.05). EB dye content in the brain tissue of the united group was less than that of model group (P<0.05). Compared with traumatic model group, concentration of malondialdehyde (MDA) decreased but concentration of total superoxide dismutase (T-SOD) in united group increased significantly (P<0.05).[Conclusion]The united group of local hypothermia and drainage shows great effect on traumatic brain edema. Its mechanism may result in inhibiting oxidative reaction and attenuating a blood-brain barrier permeability defect in the united group.

关 键 词：脑损伤/治疗 引流 低温 

分 类 号：R651.15[医药卫生—外科学]