低温胁迫对圆叶决明的伤害及施用Ca肥防护效果的研究  

Effect of chilling stress on the injury of Chamaecrista rotundifolia and protective function of applying calcium fertilizer

作　　者：翁伯琦[1] 江福英[1] 应朝阳[1] 方金梅[1] 李延[2] 

机构地区：[1]福建省农科院生态农业研究中心,福州350013 [2]福建农林大学资源与环境学院,福州350002

出　　处：《农业工程学报》2005年第6期110-113,共4页Transactions of the Chinese Society of Agricultural Engineering

基　　金：福建省科技厅重点资助项目(2003N044)

摘　　要：圆叶决明在0℃低温下分别胁迫6、12、18、24h后,叶片叶绿素总量较对照(25℃)下降21.89%、26.04%、28.16%、29.31%,可溶性蛋白质含量、H2O2含量和·O-2净产生速率随胁迫时间增加,活性氧清除酶SOD的活性随胁迫时间先上升后下降。施Ca2+处理可明显降低圆叶决明叶片·O-2净产生速率、H2O2含量和细胞质膜的电解质渗透率,同时提高了叶绿素含量和抑制活性氧的产生,其中以0.5mmol/L浓度的CaCl2处理效果为最佳,说明Ca2+对低温胁迫下圆叶决明的膜结构起有效的保护作用。After 6, 12, 18 and 24 hours' treatment with 0°C chilling stress on Chamaecrista rotundifolia, the contents of chlorophyll (chl) decreased by 21.89%, 26.04%, 28.16% and 29.31% than CK(25°C), respectively, dissolubility protein content, H2O2 content and ·O2- net generation rate increased with chilling stress time, the scavenging enzymes (SOD) activity increased at earlier stage and then dropped. The treatment of Ca2+ fertilizer under chilling stress could significantly decrease the net rate of ·O2- generation, the content of H2O2 and the leakage rate of electrolyte, the contents of scavenging enzymes SOD, meanwhile, the chlorophyll (chl) content increased. The effect of 0.5 mmol/L CaCl2 treatment was the best. The results indicated that Ca2+ could protect the membrane structure of Chamaecrista rotundifolia under chilling stress.

关 键 词：低温胁迫 圆叶决明 钙肥 叶绿素 活性氧 过氧化物歧化酶 电解质渗透率 

分 类 号：S426[农业科学—植物保护]