铁皮石斛不同生长阶段遗传稳定性的RAPD分析  

Analysis on Genetic Stability for different development stages of Dendrobium officinale Kimura et Migo by RAPD

作　　者：刘石泉[1] 李小军[1] 余庆波[1] 周根余[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2005年第2期72-76,共5页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海市高等学校科学技术发展基金项目(03DZ02).

摘　　要：利用从20个随机引物筛选出来的3个稳定性较好的10碱基引物对已继代12到13 次仍然维持在不同生长阶段、来自同一蒴果的铁皮石斛T31种群进行RAPD检测其遗传稳定性.发现不同生长阶段内遗传相似系数较高,在89.36%和100%之间,其中原球茎期的变异比萌芽期、一叶期、两叶期、开花期要显著,但程度都极其微弱.To research the hereditary stability of Dendrobium officinale Kimura et Migo which were subcultured 12 ~ 13 times in the same tissue culture system from five different development stages, 3 ten - base primers screened from 20 arbitrary primers were used for DNA amplification with random amplified polymorphic DNA (RAPD). The results showed that the genetic similarity coefficient are very high which varied from 89. 36% to 100%. The variation of protocorm were rather more remarkable than that of germination, monoleaf - stage, bileaf - stage and inflorescence.

关 键 词：遗传稳定性 生长阶段 铁皮石斛 RAPD分析 RAPD检测 遗传相似系数 引物筛选 T31 萌芽期 原球茎 开花期 继代 蒴果 

分 类 号：S511[农业科学—作物学] S828.5