水泡性口炎病毒诱导BHK-21细胞凋亡的初步研究  

Preliminary Study of Apoptosis of BHK-21 Cells Induced by Vesicular Stomatitis Virus

作　　者：褚秀玲[1] 苏建青[1] 高丰[2] 周学章[2] 成军[2] 

机构地区：[1]河北北方学院动物医学系,河北张家口075000 [2]中国人民解放军军需大学军事兽医系,吉林长春130062

出　　处：《河北北方学院学报（自然科学版）》2005年第1期68-71,共4页Journal of Hebei North University:Natural Science Edition

基　　金：国家自然科学基金资助项目 (30270994)

摘　　要：采用倒置相差显微镜、电子显微镜、琼脂糖凝胶电泳等技术研究水泡性口炎病毒诱导BHK-21细胞凋亡.形态学观察结果显示,水泡性口炎病毒感染BHK-21细胞的形态变化具有较典型的凋亡特征,如细胞膜内陷、核染色质凝集边聚等;琼脂糖凝胶电泳出现180～200 bp整倍数的DNA梯形带.上述结果表明水泡性口炎病毒可诱导BHK-21细胞凋亡.In this paper, the authors studied the apoptosis of BHK-21 infected by vesicular stomatitis virus. The morphologic results showed that BHK-21 cells infected by VSV appeared peripheral condensation of chromatin, partition of cytoplasm and nucleus into membrane and so on. In addition, agarose gel electrophoresis of DNA extracted from infected BHK-21 cells revealed typical 180-200 bp integer-fold 'ladder' bands.The results revealed that VSV could induce apoptosis in BHK-21 cells.

关 键 词：细胞凋亡 病毒诱导 琼脂糖凝胶电泳 BHK-21细胞 水泡性口炎病毒 相差显微镜 电子显微镜 形态学观察 染色质凝集 形态变化 病毒感染 细胞膜 梯形带 DNA 

分 类 号：Q255[生物学—细胞生物学] S943.112[农业科学—水产养殖]