革兰阳性菌肽聚糖对人嗜碱性粒细胞中TLR_3、TLR_9 mRNA的上调作用  

Up-regulatory Effect of Peptidoglycan on the Expressions of TLR_3 and TLR_9 mRNA in Human Basophils

作　　者：侯一峰[1] 何韶衡[1] 

机构地区：[1]汕头大学医学院变态反应与炎症学研究所,广东汕头515041

出　　处：《汕头大学医学院学报》2005年第2期74-76,共3页Journal of Shantou University Medical College

基　　金：李嘉诚基金会资助项目(C0200001)

摘　　要：目的:探讨Toll样受体(TLR)的配体肽聚糖(PGN)、聚肌苷酸_聚胞苷酸(PolyI_C)、脂多糖(LPS)和含有去甲基化的寡核苷酸序列(CpGDNA)对嗜碱性粒细胞中TLR2～4和TLR9mRNA表达的调节作用。方法:用实时定量_逆转录聚合酶链反应检测PGN、PolyI_C、LPS和CpGDNA刺激人嗜碱性粒细胞系(KU812)后,TLR2～4和TLR9mRNA表达的变化。结果:PGN刺激KU812细胞后,TLR3和TLR9mRNA的表达与未刺激组比有统计学意义(P<0.05);而PolyI_C、LPS和CpGDNA刺激KU812细胞后,细胞中TLR2～4和TLR9的mRNA与未刺激组比无统计学意义(P>0.05)。结论:革兰阳性菌的感染可能增加人嗜碱性粒细胞对病毒或其它细菌感染的敏感性。Objective:To investigate the regulatory effects of Toll like receptor(TLR)ligands[peptidonlycan(PGN)、polyinosinic_polycytidylic acid(PolyI_C)、lipopolysaccharide(LPS)and unmethylated CpG motifs(CpG DNA)]on the expressions of TLR_(2～4) and TLR_9 mRNA in human basophils.Methods:Real_time quantitative reverse transcriptase_polymerase chain reaction was used to examine the effects of PGN、Poly I_C、LPS and CpG DNA on expressions of mRNA for TLR_(2～4) and TLR_9 in basophils.Rusults:PGN strongly up_regulated the mRNA expression of TLR_3 and TLR_9,whereas Poly I_C、LPS and CpG DNA had no regulatory effects on mRNA expression of TLR_(2～4)and TLR_9.Conclusion:The infection of human basophils with gram_positive bacteria may enhance their sensitivity to virus or other bacteria.

关 键 词：嗜碱性粒细胞 TLR9 革兰阳性菌 肽聚糖 上调作用 聚肌苷酸-聚胞苷酸 脂多糖(LPS) 聚合酶链反应检测 mRNA表达 Toll样受体 核苷酸序列 去甲基化 调节作用 实时定量 粒细胞系 细菌感染 PGN CpG DNA 统计学 逆转录 IPS 敏感性 

分 类 号：R446.113[医药卫生—诊断学] R392.11[医药卫生—临床医学]