肿瘤坏死因子-α和干扰素-γ对人嗜碱性粒细胞中Toll样受体基因表达的调节作用  

Regulatory Effects of Tumor Necrosis Factor-α and Interferon-γ on the Expression of Toll Like Receptor Gene in Human Basophils

作　　者：侯一峰[1] 何韶衡[1] 

机构地区：[1]汕头大学医学院变态反应与炎症学研究所,广东汕头515041

出　　处：《汕头大学医学院学报》2005年第2期77-80,共4页Journal of Shantou University Medical College

基　　金：李嘉诚基金会资助项目(C0200001)

摘　　要：目的:探讨肿瘤坏死因子α(TNFα)和干扰素γ(IFNγ)对人嗜碱性粒细胞中Toll样受体(TLR)基因表达的调节作用。方法:用逆转录聚合酶链反应检测人嗜碱性粒细胞系(KU812)中,TLR1～10mRNA的组成型表达;用实时定量聚合酶链反应检测TNFα或IFNγ刺激KU812细胞后,TLR1～10mRNA表达的变化。结果:人嗜碱性粒细胞有TLR1～10mRNA的组成型表达,其中TLR1～6和TLR9的mRNA表达量较高,而TLR7,8和TLR10mRNA的表达量相对较少。TNFα可明显上调TLR2～4mRNA的表达,而IFNγ对TLR2和TLR9的mRNA表达有明显的上调作用。结论:人嗜碱性粒细胞不仅参与了天然免疫,而且其免疫功能还受到细胞因子的调节。Objective:To investigate the regulatory effects of tumor necrosis factor-α(TNF-α)and interferon-γ(IFN-γ)on the expression of Toll like receptor(TLR)gene in human basophils.Methods:Constitutive expression of TLR_(1～10)?mRNA in resting human basophil cell line(KU812)was examined with reverse transcriptase-polymerase chain reaction,while the alterations of TLR_(1～10)?mRNA expression in KU812 cells were quantitated using real-time quantitative-polymerase chain reaction.Rusults:KU812 cells expressed all members of the TLR family(from 1 to 10),but mainly expressed TLR_(1～6) and TLR_9.TNF-α strongly up-regulated the expression of TLR_(2～4)?mRNA and IFN-γ significantly up-regulated mRNA expression of TLR_2 and TLR_9.Conclusion:The human basophils are involved in innate immune responses and their functions can be regulated by cytokines.

关 键 词：嗜碱性粒细胞 TOLL样受体 干扰素-Γ 调节作用 基因表达 肿瘤坏死因子-α(TNF-α) 聚合酶链反应检测 mRNA表达量 组成型表达 IFN-γ TLR9 TLR2 粒细胞系 实时定量 上调作用 天然免疫 细胞因子 免疫功能 逆转录 

分 类 号：R446.113[医药卫生—诊断学] R392.11[医药卫生—临床医学]