大鼠脊髓慢性受压后脊髓组织神经生长因子及其受体的表达  

Influence of Progressive Compression on the Expression of Nerve Growth Factor and TrkA in Chronic Spinal Cord Injury of Rats

作　　者：王新家[1] 叶卫莲[1] 孔抗美[1] 蔡燕玲[1] 周雪琼[1] 齐伟力[1] 

机构地区：[1]汕头大学医学院第二附属医院关节脊柱外科,广东汕头515041

出　　处：《汕头大学医学院学报》2005年第2期86-87,F003,共3页Journal of Shantou University Medical College

基　　金：广东省医学科研基金资助项目(A2003521);广东省中医药局课题(303015);汕头大学发展基金资助

摘　　要：目的:观察大鼠脊髓慢性受压后脊髓组织中神经生长因子(NGF)及其受体(TrkA)表达的变化。方法:采用大鼠后路渐进性压迫的方法制作大鼠轻、中、重度脊髓压迫损伤动物模型,用免疫组化检测脊髓组织中NGF及其受体的表达的变化。结果:正常大鼠脊髓组织中的脊髓神经元和灰、白质中的胶质细胞NGF和TrkA表达较弱;脊髓受压后NGF和TrkA表达增强(P<0.05)。受压程度越重,其表达越明显,免疫反应强度与脊髓受压程度呈正相关。结论:大鼠脊髓慢性受压后,NGF及其受体表达增多,这可能对脊髓神经元的存活和保留具有重要作用。Objective:To study the change of the expression of nerve growth factor(NGF)and its receptor(TrkA)in chronic progressive compressive spinal cord injury of rats.Methods:A rat model of chronic spinal cord injury was made.The expressions of NGF and TrkA were detected by immunohistochemistry method.Results:The positive expressions of NGF and TrkA were observed in neurons and the glial cells in normal spinal cord of rats.After spinal cord injury,the numbers of positive neurons of NGF and TrkA increased (P<0.05).Moreover,there was a positive correlation between the positive NGF immuno-reactive density and the extent of compressed spinal cord.Conclusion:In the progress of chronic compressive spinal cord injury,the expression of NGF and TrkA increases,and that may be of benefit to the reservation and regenerations of the injured spinal cord neurons.

关 键 词：脊髓组织 慢性 神经生长因子(NGF) 脊髓神经元 免疫组化检测 TRKA 脊髓受压 动物模型 脊髓压迫 正常大鼠 胶质细胞 表达增强 反应强度 受体表达 渐进性 正相关 

分 类 号：R651.2[医药卫生—外科学] R277.7[医药卫生—临床医学]