高效液相色谱法测定血浆中拉莫三嗪浓度  

A Rapid Assay of Serum Level of Lamotrigine by Liquid Chromatography

作　　者：肖波[1] 章蓓[1] 谢光洁[1] 

机构地区：[1]湖南医科大学附属湘雅医院神经科

出　　处：《临床实用神经疾病杂志》1995年第1期23-25,共3页

摘　　要：本文报告一种用高效液相色谱快速分析抗癫痫新药拉莫三嗪的方法。３，５－二胺－６－（２甲氧苯）－ｌ，２，４一三嗪作为内标，标本经乙酸乙酯萃取，蒸干后用５０μｌ乙醇重溶，取１０μｌ进样。色谱条件是以Ａｌｔｅｘ正相色谱柱（４．６ｍｍＩＤ×７．５ｃｍ，ｄｐ３μｍ）作为分析柱，乙烷：乙醇：胺水为８０：２０：０．２５作为流动相，检测器波长置３１３ｍｍ处。结果表明本法具有快速、灵敏、选择性高等特点，完全适用于拉莫三嗪的血浓度监测。We described a sensitive,specific and fast liquid chromatographic assay for determining lamotrigine,a novel anticonvulsant drug,by using a high-speed column.Lamotrigine is extracted from l00 μl of serum with ethylacetate containing 3,5-diamino-6-(2-methoxyphenyl)-1,2,4-triazine as internal standard.The residue is reconstituted with 50μl of ethanol and 10μlof the final solution is injceted into the column,The drug and internal standard are eluted at room tempera ture with a mobile phase of n-hexane/absolute ethanol/35%amminoa(80/20/0.25,by volume)at a flow rate of 2 ml/min,monitored at 3l3 nm.Analytical recovery ranges from 92.3to 94.4%,with-run CV ranges from 4.4 to 4.8%and between-run CV from 6.0 to 605%

关 键 词：拉莫三嗪 高效液相色谱法 血浆 测定 乙酸乙酯萃取 抗癫痫新药 血浓度监测 快速分析 色谱条件 流动相 检测器 乙醇 

分 类 号：R971.6[医药卫生—药品] R286.0[医药卫生—药学]