一种制备酶标记抗体的新技术及在血痕种属鉴定的应用  

A New Method for Preparation of Enzyme-Antibody Conjugate and Its Application in Serology

作　　者：周大鹏[1] 吴文庆[1] 周月琴[1] 

机构地区：[1]上海医科大学基础医学院生物化学教研室,上海医科大学基础医学院法医学教研室

出　　处：《法医学杂志》1995年第2期55-56,共2页Journal of Forensic Medicine

摘　　要：本实验建立了一种过碘酸钠氧化法为基础、制备ＨＲＰ标记的免抗人ＩｇＧ抗体的新技术。首先以己二酰二肼作为肼化剂，在ＩｇＧ分子上接上肼基，然后以过碘酸钠作为氧化剂，使ＨＲＰ分子上的糖基氧化为醛基，进而使醛基与联结在ＩｇＧ分子上的肼基形成Ｓｃｉｆｆ氏碱而达到酶与抗体的联接。在用斑点ＥＬＩＳＡ方法鉴别血痕种属的研究中，该法制各的酶标记抗体具有高效价，高敏感度，高特异性等优点。A modified periodate oxidation method was developed to label rabbit-ant-human IgG with HRP.The antibody was first hydrazized with adipic dihydrazide,then the hydrazide groups of the antibody-hydrides reacted with the aldehydes that were formed on the sugars of HRP by periodate oxidation. The labeled antibody was utilized for species identification by dot- ELISA through detection of human immunoglobin and proved to be specialized and sensitive.

关 键 词：酶标记抗体 血痕种属 制备 抗人IgG抗体 ELISA方法 IgG分子 鉴定 过碘酸钠 高特异性 HRP 氧化法 氧化剂 高效价 敏感度 醛基 

分 类 号：R446.61[医药卫生—诊断学] R446.11[医药卫生—临床医学]