米酵菌酸中毒小鼠顶叶皮质超微结构观察  

Ultrastructure Observation of Parietal Cortex of Intoxicated Mice by Bongkrekic Acid

作　　者：吴洪娟[1] 王尊哲[1] 姜红心[1] 李锋杰[1] 

机构地区：[1]潍坊医学院形态学实验室,山东潍坊261042

出　　处：《解剖科学进展》2005年第2期136-137,140,共3页Progress of Anatomical Sciences

摘　　要：目的观察米酵菌酸对小鼠顶叶皮质毒性作用的超微结构改变,探讨米酵菌酸的中毒作用机理。方法小鼠腹腔注射米酵菌酸,2h后取其大脑皮质,常规透射电镜样品制备,电镜观察其组织的超微结构。结果米酵菌酸主要损伤皮质神经元、胶质细胞和毛细血管内皮细胞的线粒体、粗面内质网及突触等结构。可见线粒体肿胀,嵴断裂或消失,部分线粒体髓样变和空泡变性;粗面内质网数量减少;核周间隙局部增宽;血脑屏障毛细血管周间隙扩张,呈空亮的囊泡样结构,内皮细胞微绒毛减少;突触小泡减少。结论米酵菌酸可造成脑组织亚细胞结构的损伤,为米酵菌酸中毒引起的神经中毒机理提供形态学依据。Objective To observe the ultrastructure changes of intoxicated mice parietal cortex caused by bongkrekic acid (BKA) and to explore the mechanism of BKA poisoning. Methods BKA 1.5 mg/kg was injected into the peritoneal cavity of mice, morphologic changes of brain tissue were observed with electron microscope. Results BKA mainly injured the mitochondria, rough endoplasmic reticulum of neuron, neuroglial cells and endothelium cells and synapse of neurons in cortex. The ultrastructural changes were as follows: swelling, rupture or disappearance of inner cristae and vesicular degeneration of mitochondria, decrease of rough endoplasmic reticulum in number; enlargement of clearance around nucleus, expanding of clearance around capillary of blood-brain barrier, with decrease of microvillus of endothelial cells, decrease of synaptic vesicle in number. Conclusion BKA can damage the ultrastructure of nerve cells, the present results provide the morphologic basis for understanding the nerve mechanism by BKA poisoning.

关 键 词：米酵菌酸中毒 顶叶皮质 超微结构观 小鼠 毛细血管内皮细胞 粗面内质网 超微结构改变 电镜样品制备 毒作用机理 皮质神经元 线粒体肿胀 毛细血管周 亚细胞结构 毒性作用 腹腔注射 大脑皮质 电镜观察 胶质细胞 空泡变性 

分 类 号：R595.7[医药卫生—内科学] R338.6[医药卫生—临床医学]