微波快速过氧化物酶染色法与灌注法用于微循环形态学研究之比较  

Rapid Microwave Peroxidase Staining Technique Compared with Perfusion One in Microcirculatory Study

作　　者：熊正文[1] 朱森树[1] 阮士荣[1] 李春光[1] 左尚真[1] 

机构地区：[1]中国人民解放军第251医院

出　　处：《微循环学杂志》1994年第3期29-31,T002,共4页Chinese Journal of Microcirculation

摘　　要：以健康Ｗｉｓｔａｒ大白鼠为材料，对微波快速内源性过氧化物酶染色与灌注法显示微血管的方法进行了对比研究。结果，过氧化物酶组织化学法具有不用灌注，操作简单，所用时间短，对血管无扩张、破裂等人为改变的特点，保持了微血管真实的自然形态和管径大小，可以定量或半定量地判定组织器官活体时的血液循环状况。可以用于人及动物的大脑、脊髓、皮肤、耳及食管等组织内微血管形态学研究和定量分析。并对过氧化物酶显示微血管的原理和微波辐射促进染色的原理及特点进行了分析、讨论。Rapid microwave-promoting endapeneous peroxidase staining technique was compared with perfusion one in microcirculatory study of Wistar rats. Results showed this method without artificial errors such as dilation and rupture of blood vessels was more rapid, simple and natural than routine perfusion one. It can be used to evaluate semi-and quantitatively the blood circulation in the tissues and organs including the brain, spinal cord, skin, ear and esophagus, etc of human and animalsby microvascular architecture analysis, also, the mechanism and characters of microwave-promoting peroxidase, staining were discussed.

关 键 词：形态学研究 过氧化物酶染色法 灌注法 微血管定量分析 微波 微循环动力学 微血管直径 不同程度 功能状态 物质交换 调节机理 变化规律 显示方法 细胞间 密度 

分 类 号：R392.12[医药卫生—免疫学] R698.2[医药卫生—基础医学]