吗啡依赖小鼠免疫功能的变化  

CHANGES OF IMMUNE RESPONSE OF MORPHINE-ADDICTED MICE

作　　者：陆正武[1] 林志彬[1] 

机构地区：[1]北京医科大学药理学教研室,北京100083

出　　处：《中国药物依赖性通报》1994年第1期4-8,共5页

摘　　要：以饮用吗啡药水法建立小鼠依赖模型,探讨其免疫机能的改变。发现,与对照组比较,依赖小鼠腹腔巨噬细胞(PMΦ)吞噬中性红能力下降,LPS 10μg·ml^(-1)诱生的IL-1及TNF活性降低。吗啡亦抑制Con A/LPS刺激的胸腺细胞和脾细胞参入[~3H]TdR,使DNFB所致迟发型过敏反应下降。若脾细胞单独或与Con A 3μg·ml^(-1)共同培养24h,成瘾小鼠产生IL-2的能力皆明显弱于对照。但Con A活化3d的脾母细胞对重组IL-2的反应性以及产生抗SRBC的空斑形成细胞数和抗体的水平,在二组之间均未见差异。Mouse dependence model was established by drinking increasing concentrations of morphine( Mor) in tap water, with which several parameters of immune function were evaluated. It was found that thioglycollate-primed macrophages of addicted mice had a lower ability to phagocytose neutral red, and to induce interleukin - 1(IL-1) and tumor necrosis factor(TNF) production stimulated by LPS 10μg·ml-1. Moreover, Mor also suppressed Con A / LPS induced thymocyte and splenocyte proliferation of [3H] TdR incorporation, and inhibited the delayed type hy'persensitivity(DTH) reaction elicited by DNFB. Additionally, the interleukin - 2(IL-2) activity was detected to be weaker in Mor-addicted mice compared with control, when splenocytes alone or plus Con A 3μg· ml-1 were cultured at 37℃ in 5% CO2 for about 24 h. However, Mor dependence had no significant influences on responsiveness of Con A-activated splenocytes of 3 d culture to recombinant IL-2,and the production of plaque forming cells (PFC) and antibody. In summary, chronic Mor administration produced declines in macrophage function and T cell-mediated, but not humoral immune response, which may provide an explanation for the immunosuppressive effects found in opiate addicts.

关 键 词：免疫功能 吗啡依赖 小鼠腹腔巨噬细胞 [^3H]TdR 迟发型过敏反应 重组IL-2 ConA活化 免疫机能 活性降低 IL-1 胸腺细胞 共同培养 空斑形成 SRBC LPS 脾细胞 对照组 中性红 TNF 24h 细胞数 反应性 母细胞 下降 能力 

分 类 号：R285.5[医药卫生—中药学] R595.5[医药卫生—中医学]