一种简易的拟南芥幼苗微量DNA提取方法  

A Rapid-simple Method of Trace DNA Extraction from a Single Plant of Arabidopsis thaliana

作　　者：杨双[1] 阮燕晔[1] 樊金娟[1] 张立军[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,沈阳110161

出　　处：《沈阳农业大学学报》2005年第1期99-100,共2页Journal of Shenyang Agricultural University

摘　　要：报道了一种简易的拟南芥幼苗微量DNA提取方法。该方法可在常温下进行DNA提取,而且样品用量少,仅需10mg左右,用100μL重蒸水回溶DNA沉淀所获得的粗提液可直接用于PCR检测。结果表明:应用该法提取的DNA完整性好,PCR效果佳,适用于拟南芥转基因植株鉴定。A rapid and simple method of trace DNA extraction from a single seedling of Arabidopsis thaliana was presented. The DNA extraction could be carried out at room temperature with about 10mg sample. The crude DNA (the DNA precipitation was dissolved by 100ul deionized water) could be applied to PCR. The results showed that the extracted DNA was integral, and electrophoresis bands from the PCR products were very clear. Therefore, This method is suitable for identifying the transgenic plants of Arabidopsis thaliana with PCR.

关 键 词：DNA提取方法 拟南芥 微量 幼苗 PCR检测 植株鉴定 粗提液 转基因 

分 类 号：S792.14[农业科学—林木遗传育种] Q943[农业科学—林学]