高压氧治疗对大鼠脑挫裂伤后细胞凋亡和凋亡相关蛋白的影响  

The effect of HBO therapy on apoptosis and apoptosis-related genes after focal brain contusion

作　　者：陈鑫[1] 朱志安[2] 程英[1] 张红[2] 邱建华[2] 

机构地区：[1]上海中医药大学附属普陀医院(上海市普陀区中心医院)神经外科,200062 [2]上海第二医科大学附属宝钢医院神经外科

出　　处：《中华航海医学与高气压医学杂志》2005年第2期89-93,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：上海市属高校自然科学研究项目(01B01)

摘　　要：目的探讨高压氧(HBO)对创伤性脑损伤的治疗作用与对细胞凋亡及凋亡相关蛋白的影响之间的关系。方法制作SD大鼠自由落体脑挫裂伤动物模型,设立HBO治疗组和对照组,分别应用透射电镜、原位末端标记法(TUNEL)和流式细胞技术对各组动物脑挫伤灶旁和海马结构的细胞凋亡情况进行检测;使用WesternBlot和免疫组化方法,检测bcl-2和Bax蛋白在脑组织中的表达情况。结果脑外伤后存在细胞凋亡,细胞凋亡的发生在伤后24h达到高峰,HBO治疗组在伤后24h和48h细胞凋亡发生率低于相应的对照组(P<0.05);脑挫裂伤灶和海马结构bcl-2蛋白的表达均增强,HBO治疗组bcl-2的表达强于对照组(P<0.05),而Bax的表达在各组间差异无统计学意义。结论HBO治疗对创伤性脑损伤后细胞凋亡的发生及凋亡相关蛋白的表达有影响,抑制细胞凋亡很可能是HBO对创伤性脑损伤治疗作用的重要机制。Objective To investigate the relationship between the therapeutic effect of hyperbaric oxygenation (HBO) on focal brain contusion and its impact on apoptosis and apoptosis-related genes.Methods The rats were randomly assigned to one of the three groups:the control,trauma and treatment with HBO groups.Focal brain contusion was induced in the latter two groups.Apoptosis of neuron cells was evaluated with transmission electron microscopy,TUNEL and flow cytometry.The cerebral expression of bcl-2 and Bax was analyzed with Western Blot and immunohistochemical technique.Results At 24 hours in the cortex and 48 hours in the hippocampus,apoptosis rates of neuronal cells for the HBO treatment group were significantly lower than those of the control(P<0.05).The expression of bcl-2 in contusive cortex and hippocampus enhanced quite obviously,following trauma;The expression of bcl-2 in the HBO group was obviously better than that of the control(P<0.05).However there was no difference in the expression of Bax in all groups (including the control).Conclusions HBO is closely correlated with apoptosis and apoptosis-related genes after traumatic brain injury,and apoptosis inhibition by HBO might be an important mechanism for the treatment of focal brain contusion after traumatic brain injury.

关 键 词：高压氧 细胞凋亡 创伤性脑损伤 Bcl-2 BAX 

分 类 号：R651.15[医药卫生—外科学]