增强UV-B辐射和CO_2倍增的交互作用对蚕豆幼苗的保护酶活性及脂质过氧化作用的影响  

MUTUAL EFFECTS OF ENHANCED UV-B IRRADIATION AND DOUBLING CO_2 ON ACTIVITIES OF CELL DEFENSE ENZYMES AND LIPID PEROXIDATION IN BROAD BEAN SEEDLINGS

作　　者：赵广琦[1] 王勋陵[2] 张利权[1] 

机构地区：[1]华东师范大学河口海岸国家重点实验室,上海200062 [2]西北大学生命科学学院,西安710069

出　　处：《应用与环境生物学报》2005年第3期296-299,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金(No.39970126);上海市重点学科建设项目;"十五""211工程"重点学科建设子项目资助~~

摘　　要：在4.52kJm-2d-1UV-BBE的UV-B辐射和700μmolmol-1的CO2浓度人工模拟复合处理下,研究了蚕豆(Vi-ciafabaL.)幼苗的紫外吸收物含量、保护酶活性和抗氧化物质含量的变化.结果表明,在UV-B辐射和CO2的单独和复合胁迫下,紫外吸收物的含量均升高,而在复合胁迫下达到最大;超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)活性在UV-B辐射下降低,在CO2和复合胁迫下增强;丙二醛(MDA)的含量在UV-B辐射下提高,在CO2胁迫下降低,在复合胁迫下接近对照;抗坏血酸(AsA)含量在UV-B辐射下降低,在CO2和复合胁迫下增加.这表明CO2倍增能提高蚕豆对增强UV-B辐射伤害的防御能力,而蚕豆体内保护酶体系(SOD、POD、CAT)、抗氧化物质(AsA)和紫外吸收物是其在高浓度CO2下增强抗UV-B辐射能力的内在基础.By the complex treatments simulating 4.52 kJ m-2 d-1 UV-BBE of ultraviolet-B (UV-B, 280～320 nm) and 700 μmol mol-1 CO2 concentration, the responses of UV-B absorbing compounds,protective enzyme system and antioxidant in broad bean (Vicia faba L.) seedlings were investigated. The results showed that the UV-B absorbing compounds increased both under the single or complex stress of enhanced UV-B irradiation and doubling CO2, and reached highest under the complex stress. The activities of superoxide dismatase (SOD), peroxidase (POD), catalase (CAT) and phenlalanine amino lyase (PAL) exposed to enhanced UV-B irradiation were lower than those of the control, while higher under the stress of doubling CO2 and the complex stress. The content of malondialdehyde (MDA) increased under the stress of enhanced UV-B irradiation, while decreased under the stress of doubling CO2, and showed no difference between the control and the complex stress. The content of ascorbic acid (AsA) decreased under the stress of enhanced UV-B irradiation, while increased under the stress of doubling CO2, as well as under the complex stress. The results indicated that doubling CO2 could improve the protective capacity against the stress of enhanced UV-B irradiation, and the protective enzyme system (SOD, POD and CAT), antioxidant (AsA) and UV-B absorbing compounds of broad bean were the intrinsic basis for this protective capacity. Fig 1, Ref 24

关 键 词：UV-B辐射 CO2倍增 保护体系 蚕豆 

分 类 号：Q947[生物学—植物学]