地鼠气管上皮损伤后粘液分泌细胞的修复作用  

Repairing Action of Mucous-Secretory Cells after Injury of Tracbeal Epithelium in Hamsters

作　　者：王恩华[1] 张丽红[1] 邱雪杉[1] 赫明昌[1] 刘闺男[1] 傅志民 

机构地区：[1]中国医科大学基础医学院病理教研室

出　　处：《解剖科学进展》1996年第2期179-184,共6页Progress of Anatomical Sciences

基　　金：国家自然科学基金

摘　　要：用改造后的静脉留置针将地鼠气管腹侧面约１／４的粘膜刮除，取材前１ｈ腹腔内注入Ｂｒｄｕ气管标本经ＨＥ、ＰＡＳ，抗Ｂｒｄｕ免疫染色，光、电镜观察。损伤后６ｈ开始，边缘部上皮细胞增殖并向损伤面内爬行，胞浆呈ＰＡＳ阳性，核呈Ｂｒｄｕ阳性，２４ｈ覆盖损伤面。电镜下这些细胞表面无纤毛或仅有少量微绒毛，浆内有大小不等散在的粘液分泌颗粒和较多的粗面内质网。３６ｈ开始，损伤面由３一５层表皮样化生细胞构成，核周出现张力丝状物围绕，部分胞浆内仍有少量粘液颗粒。从７２ｈ开始，细胞出现短而细的纤毛或出现较多的粘液分泌空泡。２ｗ后恢复正常气管上皮结构。这表明气管上皮中的粘液分泌细胞不是终末细胞，具有很强的ＤＮＡ合成和增殖能力，是损伤后参与修复的主要细胞之一。bout one fourth of the tracheal mucosa of hamster was denuded by a venous needle reformed. Atone hour prior to killing each hamster was given a single intraneritoneal injecton of bromodeoxyuridineBrdu. tracheal specimens were stained with HE. PAS and anti-Brdu immunohistochemistry, then ob-served under the light and electron microscope. From 6h post injury the cells regenerated and migratedfrom the margin to wound site and the wound area was completely covered by a single layer of non-cili-ated flatted cells. Most of their nuclei were Brdu (+) and cytoplasms were PAS( + ) ,some mucous se-cretory granules and rich rER were observed. At 36h , 3-5 layers of epidermoid metaplasia cell: coveredall of the wound area and lots of tonofilament was observed around their nuclei. a few mucous granulesalso were seen in their cytoplasm. At 72h . there were short and small ciliates or many mucous granulesin theose cells. When 2 weeks the epithelium recovered the normal epithelial architecture. The resultsindicated that mucous secretory cells is not the end-stage cell . the ability to synthesize DNA and re-generate, but one of main cells of repairing tracheal epithelium after injury.

关 键 词：地鼠 上皮损伤 粘液分泌细胞 修复作用 气管粘膜 

分 类 号：R322.33[医药卫生—人体解剖和组织胚胎学]