一氧化氮对肝硬化大鼠全胃肠外营养时肝脏损害的保护作用  

Study on effects of NO on hepatic injury of cirrhotic rats with TPN

作　　者：梁力建[1] 罗时敏[2] 

机构地区：[1]中山医科大学附属第一医院肝胆外科,广州市510080 [2]江西省人民医院外科

出　　处：《中国临床营养杂志》2000年第2期92-95,共4页Chinese Journal of Clinical Nutrition

摘　　要：目的　探讨一氧化氮在硬化肝全胃肠外营养 (TPN)时进一步肝损害中的作用。　方法　 2 6只肝硬化大鼠随机分成 4组 ,TPN组 7只 ,TPN +L Arg组 6只 ,TPN +L NAME组 6只 ,TPN +L Arg +L NAME组 7只。测定肝功能、肝甘油三酯 (TG)、胆固醇 (CHO)含量、血清NO浓度、肝脏NO含量、肝脏NOS比活性 ,观察肝脏 β NADPH黄递酶组化染色及肝脏病理组织学改变的情况。　结果　TPN组、TPN +L NAME组和TPN +L Arg +L NAME组各有一只大鼠因输液过快 ,肺水肿死亡 ,其余大鼠均成活。与TPN组比较 ,TPN +L Arg组血清NO浓度、肝脏NO含量明显升高 (P <0 .0 5 ) ,而血清AST明显降低 (P <0 .0 5 ) ,ALT降低但无统计学意义 ,肝TG含量明显降低 (P <0 .0 5 ) ,肝脂肪变性程度减轻。TPN +L NAME组血清NO浓度、肝脏NO含量明显降低 (P <0 .0 5 ) ,而血清AST、ALT明显升高 (P <0 .0 5 ) ,肝TG含量明显升高 (P <0 .0 5 )。各组血清ALB、A/G比值无明显差别。各组肝脏NOS比活性无明显差别 (P >0 .0 5 ) ,β NADPH黄递酶组化染色为 ++。　 结论　一氧化氮在硬化肝全胃肠外营养时的进一步肝损害有保护作用。Objective To investigate the effects of nitric oxide (NO) on further hepatic injury of cirrhotic rats with total parenteral nutrition (TPN). Methods 26 cirrhotic rats are divided into four groups randomly:TPN group ( n =7),TPN+L-Arg group ( n =6),TPN+L-NAME group ( n =6) and TPN+L-Arg+L-NAME group ( n =7).Liver function,hepatic triglycerin (TG) and cholesterol (CHO),the level of serum and liver NO,liver nitric oxide synthase (NOS) activity were measured.β-NADPH diaphorase statining and histologic changes of the liver were observed. Results All animals survived but one rat died of pneumonedeman in TPN group,TPN+L-NAME group and TPN+L-Arg+L-NAME group respectively.Compared with TPN group,In the TPN+L-Arg group,the level of serum and liver NO were increased significantly ( P <0.05),however,serum AST was decreased significantly ( P <0.05) but ALT was not,liver TG was decreased significantly ( P <0.05) and the degree of fatty degeneration in the liver was lessened.In the TPN+L-NAME group,the level of serum and liver NO were decreased significantly ( P <0.05),however,serum AST and ALT were increased significantly ( P <0.05),the level of liver TG was increased significantly ( P <0.05).Serum ALB,A/G ration and liver NOS activity had not significantly changes among all groups.The degree of β-NADPH diaphorase staining was ++. Conclusions NO may play a protective role in further liver injury of cirrhotic rats with TPN.

关 键 词：全胃肠外营养 一氧化氮 保护作用 肝硬化 大鼠 肝脏损害 肝脏病理组织学改变 甘油三酯(TG) 酶组化染色 血清AST NO浓度 NO含量 A/G比值 TPN 输液过快 变性程度 肝损害 比活性 NOS ALT 肝功能 胆固醇 肺水肿 升高 统计学 

分 类 号：R459.3[医药卫生—治疗学] R575.2[医药卫生—临床医学]