实验性早期心肌梗死的肌红蛋白免疫组织化学研究  

IMMUNOHISTOCHEMICAL STUDY 0F MYOGLOBIN IN EXPERIMENTAL EA-RLY MYOCARDIAL INFARCTION

作　　者：姚季生[1] 姚申[1] 应爱娣[1] 颜召文[1] 佘伟良[1] 

机构地区：[1]上海第二医科大学法医学教研室,上海200025

出　　处：《法医学杂志》1992年第2期49-51,F002,共4页Journal of Forensic Medicine

摘　　要：结扎大鼠冠状动脉前降支,制造早期(5分钟到24小时)心肌梗死模型.用抗肌红蛋白抗体免疫组织化学技术(PAP法)观察大鼠心肌梗死区肌红蛋白脱失(缺染)情况,结果表明:冠状血管结扎后30分钟,梗死区少数心肌出现轻度不完全性缺染;结扎2-6小时,梗死区心肌缺染范围扩大呈带状、条状、片状;结扎lO-24小时,梗死区心肌缺染范围达全层,大部分属完全性缺染,与非梗死区分界清晰.We produced the animal model of the early myocardial infarction (5 min to 24 h) by ligating the left anterior descending coronary artery of-rats. The immunohistochemical methods (PAP) of myoglobin antibody were performed to observe the myoglobin defects of myocytes in infarcting area of rat hearts. The results demonstrated that: Slight imcomplete myoglobin defects in few myo-cytes of infarcting area were noted at 30 min after coronary ligation. At 2-6h after coronary ligation, the limits of the myoglobin defects was extended and become ribbon strip or sheet in shape. At 10- 24h after ligation, the myoglobin defects of myoc-ytes in infarcting area attain total layer of myoc-ardium, the majority of myoglobin defects was complete and could be clearly differentieted from the non-infarcting area.

关 键 词：免疫组织化学研究 肌红蛋白 早期心肌梗死 实验性 免疫组织化学技术 冠状动脉前降支 心肌梗死模型 大鼠心肌 PAP法 蛋白抗体 冠状血管 不完全性 非梗死区 心肌缺 结扎后 

分 类 号：R654.2[医药卫生—外科学] R542.22[医药卫生—临床医学]