上海地区中国人红细胞GPT表型频率调查  

Investigation on the frequencies of the Red Cell GPT Phenotypes in Shanghai district

作　　者：吴文庆[1] 陈磊[1] 张其英[1] 

机构地区：[1]上海医科大学法医学教研室

出　　处：《法医学杂志》1991年第2期9-11,共3页Journal of Forensic Medicine

摘　　要：谷丙转氨酶(Glutamic-Pyruvic ransminas，GPT)是参于机体氨基酸代谢的一类酶，广泛存在于机体各组织中。GPT有两种形式．一种为线粒体型GPT(mGPT)，存在干线粒体中，另一种为可溶性GPT(sGPT)，存在于胞浆中。可溶性GPT在肝细胞、心肌细胞中含量较高，红细胞中亦含有一定量具有活性的可溶性GPT。Genetic polymophism of Soluble glutamic-pyruvic fransminase(GPT)was discoverd by Chen and Gib- lett with Starch-gel electrophoresis of human red cells.Three Common phenotypes,designated GPT_1.GPT_(2-1) and GPT_2,were identified and each was thought to be the expression of two alleles Gpt^1 and Gpt^2 at autosomal locus. In this study we have investigated the polymor-phism of GPT in 235 normal unrelated donors living in Shanghai.Enzyme typing was carried out for Starchgel and specific enzyme Staing.The gene frequen- cies of Gpt^1 and Gpt^2 were estimated at 0.5404 and 0.4596.The observed numbers of the phenotypes were in good agreement with the numbers expected under the Hardy-Weinberg equelihrium(x^2=1.9939 0.1<p <0.2). In this Study We improved the method of enzyme Staing by using filter paper instead of agarose to make the result more observable and to keep it for a langer time.

关 键 词：上海 中国人 红细胞 GPT 表型频率 调查 肝细胞 心肌细胞 

分 类 号：D919.2[医药卫生—法医学]