BMP-2基因转染的自体骨髓间充质干细胞修复羊股骨头坏死的生物力学研究  

Biomechanical study on the repair of femoral head necrosis in goat with the BMP-2 gene transfected autologons bone marrow derived mesenchymal stem cells

作　　者：陆斌[1] 汤亭亭[1] 岳冰[1] 戴尅戎[1] 楼觉人[2] 

机构地区：[1]上海第二医科大学附属第九人民医院骨科,上海200011 [2]美国华盛顿大学医学院骨科

出　　处：《医用生物力学》2005年第2期71-75,共5页Journal of Medical Biomechanics

基　　金：上海市科委重点课题(024119016)

摘　　要：目的观察BMP-2基因转染的自体骨髓间充质干细胞(MSCs)修复羊股骨头坏死后的局部生物力学变化。方法11只羊共22侧后肢,其中3只(6侧)作为正常对照,8只(16侧)通过结扎旋股内外侧动脉和液氮灌注等方法建立股骨头无菌性坏死动物模型。8只模型羊中,3只(6侧)不处理作为空白对照;5只共10侧植入分别复合BMP-2基因或β-gal基因转染的自体MSCs的β-磷酸三钙多孔材料,其中左侧为BMP-2治疗组,右侧为β-gal对照组,植入后第16周将所有动物处死,取植入区松质骨标本和软骨下骨进行压缩试验。结果BMP-2组植入区松质骨标本的最大抗压强度为(38.35±4.16)MPa,弹性模量为(86.85±7.28)MPa;软骨下骨的最大抗压强度为(80.76±5.32)MPa,弹性模量为(126.65±10.88)MPa。这些参数均与正常骨接近,显著大于未治疗组和β-gal组(均为P<0.05)。结论BMP-2基因转染自体干细胞可促进坏死股骨头修复,改善植入区松质骨和软骨下骨的力性能,防止股骨头软骨面的塌陷。Objective To study the change of local biomechanical property after treatment of femoral head necrosis with BMP-2 gene therapy. Methods 11 goats with 22 sides were divided into 4 groups: Ⅰnormal group (n=6 ), Ⅱ untreated group (n=6 ), Ⅲ β-gal group (n=5), Ⅳ BMP-2 group (n=5). TCP (β-tricalcium phosphate) loaded with Adv-βgal or Adv-hBMP-2 gene transfected MSCs was implanted into femoral head at 3rd week after establishment of animal models of femoral head necrosis. All goats were killed at 16th week after treatment. Sub?-chondral bone and cancellous bone were harvested to test its biomechanical property. Results The maximum stiffness and elastic modulus of cancellous bone in BMP-2 group were (38.35±4.16) MPa and (86.85±7.28) MPa, respectively. The maximum stiffness and elastic modulus of sub-chondral bone in BMP-2 group were (80.76±5.32) MPa and (126.65±10.88) MPa, respectively. The parameters in BMP-2 group are higher than that both in β-gal group and untreated group(P<0.05), but close to normal group. Conclusion The BMP-2 Gene transfected autologous bone marrow-derived mesenchymal stem cells can accelerate the repair of femoral head necrosis and prevent the collapse of femoral head.

关 键 词：股骨头坏死 骨形态发生蛋白-2 基因转染 骨髓间质干细胞 磷酸三钙 最大抗压强度 弹性模量 

分 类 号：R318.01[医药卫生—生物医学工程]