白藜芦醇抑制胆囊癌细胞生长与诱导细胞凋亡的实验研究  

Research on Resveratrol’s Effects on Suppressing Growth and Inducing Apoptosis of GBC Cells

作　　者：胜利[1] 安利峰[2] 何烨[2] 范桂香[1] 袁育康[1] 

机构地区：[1]西安交通大学医学院免疫学教研室,西安710061 [2]西北民族大学医学院,兰州730030

出　　处：《中药材》2005年第6期489-491,共3页Journal of Chinese Medicinal Materials

摘　　要：目的探讨白藜芦醇(Res)对胆囊癌细胞(GBC)和正常成纤维细胞(3T3)体外增殖的影响,进而观察Res对GBC和3T3细胞凋亡的影响。方法MTT法测定肿瘤细胞生长抑制率;流式细胞术分析细胞周期,检测细胞凋亡;SABC法检测细胞bcl2、cmyc、p53蛋白表达。结果Res呈浓度依赖性抑制GBC细胞的生长与增殖(P<0.01),抑制率最高可达54%。Res能明显诱导GBC细胞凋亡,凋亡率最高为30.52%;处理组较对照组G1期细胞由34.88%上升至55.47±3.95%,S期细胞减少8.41%～17.54%,呈明显的G0/G1期阻滞现象。GBC细胞的bcl2、cmyc基因蛋白表达降低,而p53基因蛋白表达增强。结论Res能通过诱导GBC细胞凋亡而抑制其生长与增殖,但对3T3细胞无此作用。Objective:To explore the contribution of resveratrol (Res) to the proliferation and apoptosis of gallbladder carcinoma (GBC) cell line as well as fibroblast 3T3 cell line in vitro.Methods:The tumor cell growth repressing rate was measured by the MTT method,and flow cytometry was used to analyse cell cycle. The gene expression of bcl-2、c-myc、p53 were examined by SABC.Results:Res obviously suppressed the proliferation(P<0.01) in concentration and time dependent manner, it’s tiptop was 54%. Res could induce apoptosis of tumor cells.The maximum apoptosis rate of tumor was 30.52%,experiment group compared with comparison group Gl’s cells rised from 34.88% to 55.47±3.95%,S’s cells reduced 8.41～17.54%,which showed obvious phenomenon of G_0/G_1 blocking.The GBC cell’s bcl-2、c-myc gene reduced expression,while p53 gene increased expression. Conclusion:The results of this study confirm the ability of Res to suppress the proliferation of gallbladder carcinoma (GBC) cell with a typical apoptotic feature in vitro.Therefore,Res may be considered as a possible treatment strategy for gallbladder carcinoma.

关 键 词：白藜芦醇 诱导细胞凋亡 癌细胞生长 实验研究 瘤细胞生长抑制率 G0/G1期阻滞 p53蛋白表达 P53基因蛋白 bcl-2 成纤维细胞 胆囊癌细胞 流式细胞术 SABC法 浓度依赖性 3T3细胞 Res GBC 体外增殖 MTT法 细胞周期 细胞减少 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]