梯度洗脱法测定麻黄及其复方制剂中麻黄类生物碱的含量  

Detection of ephedrine and pseudoephedrine in ephedra herb and its compound preparations by high-performance liquid chromatography

作　　者：霍务贞[1] 姜红宇[1] 朱盛山[1] 

机构地区：[1]广东药学院中药开发研究所,广东广州510240

出　　处：《广东药学院学报》2005年第3期249-251,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：国家中医药管理局中医药科学技术研究基金项目(00-01LP12);广东省重点科技攻关项目(C30107)

摘　　要：目的用反相高效液相色谱梯度洗脱法测定麻黄及其复方制剂中麻黄类生物碱的含量。方法用AgilentDAD二元泵高效液相色谱仪,色谱柱EclipseSBC18(4.6mm×150mm,5μm),检测波长210nm,流速1.0mL/min,流动相为甲醇-磷酸缓冲盐溶液梯度洗脱。结果麻黄碱和伪麻黄碱在进样量分别为1.01～10.1μg、0.97～9.7μg范围内进样量与峰面积呈良好的线性关系。该方法麻黄碱平均回收率为100.14%(n=9),伪麻黄碱平均回收率为99.16%(n=9)。结论方法简便,专属性好,可以用于麻黄药材及其复方制剂的含量测定。Objective To determine simultaneously the contents of ephedrine and pseudoephedrine in ephedra herb and its compound preparations by high-performance liquid chromatography. Method The detection was performed on a reversed-phase C_ 18 column(4.6 mm ×150 mm,5 μm)at the wavelength of 210 nm. The mobile phase (methanol -0.01 mol/L phosphate buffer, pH 5.8) in gradient elution was pumped at a flow rate of 1.0 mL/min. Results The assay exhibited a linear range of 1.01～10.1 μg for ephedrine and 0.97～9.7 μg for pseudoephedrine. The average recoveries were 100.14% and 99.16% for ephedrine and pseudoephedrine, respectively. Conclusion The method is rapid, accurate and reliable for the quality control of ephedra herb and its compound preparations.

关 键 词：高效液相色谱法 梯度洗脱 麻黄碱 伪麻黄碱 

分 类 号：R284[医药卫生—中药学]