α-IFN质粒DNA-脂质体制剂的制备及其在皮肤中表达的研究  

Preparation of α-IFN plasmid DNA-liposome formulation and study on its transgenic expression in skin following topical application

作　　者：苏薇薇[1] 钟雪云[2] 周慧[1] 邓例华[2] 杨立伟[1] 陈丽云[1] 杨丽平[1] 方铁铮[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]暨南大学医学院,广东广州510632

出　　处：《广东药学院学报》2005年第3期279-281,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：广州市科技计划项目(编号2000Z02101)

摘　　要：目的研制α干扰素(αIFN)质粒DNA脂质体制剂,为临床提供一种经病灶部位透皮给药后转基因表达抗乳头瘤病毒(HPV)的新制剂。方法利用基因克隆技术和脂质体制备技术,得到在生物体细胞内表达αIFN的表达载体,含有质粒DNA的脂质体制剂;利用ELISA方法测定在皮肤细胞层中αIFN的表达水平。结果连续3天经皮给药脂质体制剂后24hpcIFN的表达水平最高;最后一次给药后连续5天αIFN的表达水平高于对照组(P<0.005);没有检测到用药组和对照组动物血清中含有αIFN。Objective Preparation of α-IFN plasmid DNA-liposome formulation to provide a novel formulation to therapy HPV following transdermal transgenic expression. Methods With subcloning and preparation of liposome techniques,the pcIFN plasmid was obtained and the plasmid-liposome formulation was made. Expression plasmid DNA for the human α-IFN protein was formulated with nonionic:cationic (NC) liposomes and applied to the auricular skin of rat in single and multi-dose protocols. The successful transfection of perifollicular cells was quantified by ELISA. Results Skin treated for 3 days with the NC liposomes had statistically significant levels of transgenic α-IFN levels present for 5 days post-treatment. Expression of transgenic α-IFN was specific for areas of skin treated with NC liposomes but not control liposome. No detectable level of expressed α-IFN was found in supernatant of animal blood. Conclusion The results indicate that the NC liposomes can delivery expression plasmid DNA to perifollicular cells and mediate transient transfection in vivo.

关 键 词：Α-干扰素 脂质体 基因治疗制剂 

分 类 号：TQ460.1[化学工程—制药化工]