山东日照和福建厦门沿海花鲈遗传差异的同工酶和RAPD分析  

The analysis of genetic variation of Lateolabrax japonicus from Rizhao of Shandong and Xiamen of Fujian by isozyme and RAPD methods

作　　者：李明云[1] 赵明忠[1] 钟爱华[1] 薛良义[1] 

机构地区：[1]宁波大学生命学院海洋与水产系,渐江宁波315211

出　　处：《海洋学报》2005年第3期119-123,共5页

基　　金：浙江省自然科学基金资助项目(300279);国家高技术研究发展计划("863")项目(2001AA623020).

摘　　要：采用聚丙烯酰胺凝胶电泳技术,对来自山东日照和福建厦门沿海花鲈的LDH,MDH,ME,αGPDH,ADH,SDH,SOD,G6PDH,EST,GDH等10种同工酶进行检测,共检测出19个基因座位,其中ME1,G6PGH2,EST1,EST5等4个基因座位具有多态性,多态座位比例均为21.1%,两群体的平均杂合度期望值分别为0.1112及0.0939;两群体间的遗传相似度和遗传距离分别为0.9902和0.0098.采用RAPD技术分析了两种群花鲈,从31条随机引物共检测出日照群体263个位点,其中具有多态性的位点占82个,多态位点比例为31.18%;检出厦门群体192个,多态位点46个,多态位点比例为24.08%;两群体间的遗传相似度为0.8338,遗传距离为0.1662.两地花鲈在基因组DNA水平上存在较大的遗传差异.利用随机引物S11,S42,S45,S52和S203,在花鲈两群体间扩增出8条稳定、明显的群体间特异性标记带,为花鲈苗种鉴别、良种培育和种质资源保护提供了遗传依据.The sea bass(Lateolabrax japonicus), captured from the coastal seas of Xiamen Fujian and Rizhao of Shandong, was sampled for the study. Polyacrylamide gel electrophoresis was used to detect the expression of isozymes in two tissues, muscle and liver. Altogether,10 isozymes including LDH,MDH,ME,α-GPDH,ADH,SDH,G-6-PDH,EST,GDH were analyzed. Biochemical genetic analysis shows that the 10 isozymes are coded by 19 gene loci, four of which are polymorphic(ME-1,G-6PGH-2,EST-1,EST-5). The percentage of polymorphic loci is 21.1%. Average heterozygosity of Rizhao stock is 0.111 2, and Xiamen stock 0.093 9. Genetic similarity and genetic distance between two stocks are respectively 0.990 2 and 0.009 8. RAPD method was also used to analyze the genetic diversity of two stocks. The result shows that using the 31 random primers, 263 RAPD sites are detected from Rizhao stock, 82 of which are genetic polymorphism, the percentage of polymorphic loci is 31.18%. 192 RAPD sites are detected in Xiamen stock, 46 of which are polymorphic, the percentage of polymorphic loci is 24.08%. The intra-stock genetic similarity between two stocks is 0.833 8, genetic distance 0.166 2. These adequately show that there is a significant genetic variation between two stocks. Specific DNA fingerprints are obtained by primers of S-11, S-42, S-45, S-52 and S-203. 8 proper bands are so obviously that might be used as molecular markers for distinguishing the different fries, also providing the genetic foundation for breeding seed high-quality seed and protecting the germplasm resource of L.japonicus.

关 键 词：花鲈 同工酶 RAPD 遗传差异 分子标记 

分 类 号：Q953[生物学—动物学] Q523