热休克蛋白70对大鼠心脏停搏供体肝移植的保护作用  

Protective effects of HSP70 on graft in rat liver transplantation from heart arrest donors

作　　者：赵雪松[1] 董春钰[1] 杨志明[1] 赵继学[1] 张学颖[1] 

机构地区：[1]吉林大学第一医院儿外科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2005年第3期358-360,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省计委科技攻关项目资助课题 (1997- 6 5 )

摘　　要：目的:采用心脏停搏供体大鼠原位肝移植模型,观察热休克蛋白70 (HSP70 )对心脏停搏后不同时间的供肝缺血再灌注损伤的影响。方法:以缺血预处理方法诱导大鼠肝脏组织产生HSP70 ,以预处理(IP)与否及供体心脏停搏4 5 m in或6 0 min的不同,将供体大鼠分为4组(IP- 4 5 ,IP- 6 0 ,C- 4 5和C- 6 0 )。于肝移植术后1h采血检测肝功能,术后1h及7d切取肝脏,观察肝脏病理学改变,并观察各组大鼠术后存活时间。结果:缺血预处理后2 4～4 8h,肝组织HSP70表达明显增强;肝移植术后1h,血清AL T和AST水平IP组明显低于对照(C)组(P<0 .0 5 ) ;术后7d,IP组移植肝光学显微镜下肝小叶结构完好,汇管区有少量淋巴细胞浸润,C组移植肝则出现了较多的肝细胞坏死和气球样变。C- 4 5和C- 6 0组1周存活率分别为12 .5 %和0 ;而IP- 4 5和IP- 6 0组1周存活率分别为6 2 .5 %和37.5 %。结论:HSP70明显改善术后肝脏功能和减轻肝脏病理学改变,显著提高心脏停搏供体肝移植的存活率,HSP70对心脏停搏供体肝移植缺血再灌注损伤具有明显保护作用。Objective To build the model of ortholopic liver transplantation (OLT) of heart arrest donor of rat to observe the effects of HSP70 on the liver graft ischemia-reperfusion injury at different time-point after heart arrest. Methods The rats were divided into 4 groups according to the ischemic preconditioning (IP) or not, and the time of heart arrest (45 or 60 min) . One hour after portal vein reperfusion,the blood samples were obtained to determine the levels of alanine amino transferase (ALT) and L-asparate amino transferase (AST), grafts samples were obtained for optical microscope observation, and the survival rate of 1 week was observed. Results The HSP70 expressions in IP groups were low at 6 h, and high at 24 h and 48 h. While there were almost no HSP70 expressions in the control group (C group) at 24 h and 48 h.At 1 h after portal vein reperfusion, the levels of ALT and AST in the IP groups (IP-45, IP-60) were significantly lower than those in C groups (C-45, C-60). At one week after the operation, in the IP groups, the structures of the hepatic lobules were very well, there were a little infiltration of lymphocytes in the portal area; while in C group there existed considerable hepatic necrosis and ballooning degeneration. In the IP groups, the survival rates at one week after operation were 62.5% and 37.5%,respectively, while in the C-45 and C-60 groups were 12.5% and 0,respectively. Conclusion HSP70 not only significantly improve liver function and graft pathological changes, but also increase survival rate of rat liver transplantation from heart arrest donors. HSP70 have remarkably protective effects on graft ischemia reperfusion injury in rat liver transplantation from heart arrest donors.

关 键 词：热休克蛋白70 心脏停搏 人工 肝移植 

分 类 号：R657.3[医药卫生—外科学] R-332[医药卫生—临床医学]